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首页> 美国卫生研究院文献>International Journal of Environmental Research and Public Health >Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids
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Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids

机译：肉桂废料的酶水解产物作为类胡萝卜素微生物生产的原料

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摘要

In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules.
机译：在全球需要走向循环经济的背景下，由于他们使用来自农业工业部门的侧流生物量来获得额外产品的微生物细胞工厂可以使用微生物细胞工厂。残留物的储存允许更好地完全使用自然资源，同时避免废物管理以满足我们的需求。在这项工作中，我们将注意力集中在多酚萃取（C-PEW）（C Cinnamomum Verum J.presl）后的肉桂废料的微生物储存，通常丢弃而没有任何额外的处理。通过酶水解释放在C-PEW中的糖，比酸水解更相容，随后的微生物培养。我们证明，尽管存在肉桂酸的抗微生物化合物存在，酵母罗达孢子脒能够生长并产生高达2.00（±0.23）Mg / L类胡萝卜素作为唯一的碳和氮源。为了进一步延长我们发现的潜力，我们测试了其他真菌细胞工厂以获得同一媒体的增长。总体而言，这些结果正在开辟基于C-PEW和微生物生物转化的单独水解和发酵（SHF）生物加工的可能性，以获得高价值分子。

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