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首页> 美国卫生研究院文献>Iranian Journal of Pharmaceutical Research : IJPR >Differential Attachment of Pulmonary Cells on PDMS Substrate with Varied Features
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Differential Attachment of Pulmonary Cells on PDMS Substrate with Varied Features

机译：不同特征对PDMS衬底对PDMS基板的差异附着

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摘要

Cancer is now a global concern, and control of the function of cancer cells is recognized as an important challenge. Although many aggressive chemical and radiation methods are in practice to eliminate cancer cells, most of them imply severe adverse toxic effects on patients. Taking advantage of natural physical differences between cancer and normal cells might benefit the patient with more specific cytotoxicity and fewer adverse effects. Physical factors are the main means that can influence cell-biomaterial interaction. To explore the importance of attachment phenomena on cancer cells in this research, polydimethylsiloxane (PDMS) substrates with varied stiffness and roughness were synthesized and lung cancer cell’s behavior on these surfaces was examined. To achieve diverse surface topography SDBD plasma was used at various exposure times, and different stiffness was obtained by changing in curing agent amount. Atomic force microscopy (AFM) and tensile modulus were employed to the characterization of roughness and stiffness respectively. Lung cancer cell survival and growth were studied by MTT and image processing analysis. The results indicated that softer and rougher surface made lung cancer cells to die. The number of detached cells, mean space of the detached cells, cellular coverage of surface, and the ratio of detached/ all cellular coverage were significantly affected by roughness and stiffness. Therefore, physical factors can control cell function, especially in lung cancer cells and these results might provide a strong base to help cancer cell removal.
机译：癌症现在是一个全球担忧，并控制癌细胞功能被认为是一个重要的挑战。虽然许多侵略性化学和辐射方法实际上是消除癌细胞，但大多数人都意味着对患者的严重不良毒性作用。利用癌症和正常细胞之间的自然物理差异可能使患者具有更具体的细胞毒性和更少的不良反应。物理因素是可以影响细胞生物材料相互作用的主要手段。为了探讨该研究中癌细胞上附着现象的重要性，合成了具有变化刚度和粗糙度的聚二甲基硅氧烷（PDMS）底物，并检查了这些表面上的肺癌细胞的行为。为了实现不同的表面形貌，在各种曝光时间使用SDBD等离子体，通过在固化剂的量改变时获得不同的刚度。原子力显微镜（AFM）和拉伸模量分别用于分别表征粗糙度和刚度。通过MTT和图像处理分析研究了肺癌细胞存活率和生长。结果表明，更软，粗糙的表面使肺癌细胞死亡。脱离细胞的数量，分离的细胞的平均空间，表面细胞覆盖率以及脱离/所有细胞覆盖的比率受粗糙度和刚度的显着影响。因此，物理因素可以控制细胞功能，特别是在肺癌细胞中，这些结果可能提供强大的基础，以帮助癌细胞去除。

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