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Temperature and Humidity PID Controller for a Bioprinter Atmospheric Enclosure System

机译：用于生物处理大气外壳系统的温度和湿度PID控制器

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摘要

Bioprinting is a complex process, highly dependent on bioink properties (materials and cells) and environmental conditions (mainly temperature, humidity and CO2 concentration) during the bioprinting process. To guarantee proper cellular viability and an accurate geometry, it is mandatory to control all these factors. Despite internal factors, such as printing pressures, temperatures or speeds, being well-controlled in actual bioprinters, there is a lack in the controlling of external parameters, such as room temperature or humidity. In this sense, the objective of this work is to control the temperature and humidity of a new, atmospheric enclosure system for bioprinting. The control has been carried out with a decoupled proportional integral derivative (PID) controller that was designed, simulated and experimentally tested in order to ensure the proper operation of all its components. Finally, the PID controller can stabilize the atmospheric enclosure system temperature in 311 s and the humidity in 65 s, with an average error of 1.89% and 1.30%, respectively. In this sense, the proposed atmospheric enclosure system can reach and maintain the proper temperature and humidity values during post-printing and provide a pre-incubation environment that promotes stability, integrity and cell viability of the 3D bioprinted structures.
机译：BioPlinting是一种复杂的过程，高度依赖于生物监测过程中的生物源（材料和细胞）和环境条件（主要是温度，湿度和二氧化碳浓度）。为了保证适当的蜂窝活力和准确的几何形状，必须控制所有这些因素。尽管包括印刷压力，温度或速度等内部因素，但在实际的生物制剂中受到良好控制的，但缺乏控制外部参数，例如室温或湿度。从这个意义上讲，这项工作的目的是控制用于生物印刷的新的大气外壳系统的温度和湿度。该控制已经通过设计，模拟和实验测试的解耦比例积分衍生物（PID）控制器进行，以确保其所有组件的正常操作。最后，PID控制器可以在611秒和湿度下稳定大气外壳系统温度，平均误差分别为1.89％和1.30％。从这个意义上讲，所提出的大气外壳系统可以在后印后达到和维持适当的温度和湿度值，并提供预孵育环境，促进3D生物印刷结构的稳定性，完整性和细胞活力。

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