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首页> 美国卫生研究院文献>Molecules >Combined Effects of Methyldopa and Flavonoids on the Expression of Selected Factors Related to Inflammatory Processes and Vascular Diseases in Human Placenta Cells—An In Vitro Study
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Combined Effects of Methyldopa and Flavonoids on the Expression of Selected Factors Related to Inflammatory Processes and Vascular Diseases in Human Placenta Cells—An In Vitro Study

机译：甲基甲戊类药物和黄酮类化合物对人胎盘细胞炎症过程和血管疾病相关的选择因素 - 体外研究

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The aim of the study was to investigate combined effects of flavonoids (apigenin, baicalein, chrysin, quercetin, and scutellarin) and methyldopa on the expression of selected proinflammatory and vascular factors in vitro for prediction of their action in pregnancy-induced hypertension. The research was conducted on a trophoblast-derived human choriocarcinoma cell line and a primary human umbilical vein endothelial cell line. Cytotoxicity of compounds in selected concentrations (20, 40, and 100 µmol) was measured using the MTT test and the concentration of 40 µmol was selected for further analysis. Subsequently, their effects with methyldopa on the expression of selected markers responsible for inflammation (TNF-α; IL-1β; IL-6) and vascular effects (hypoxia-inducible factor 1α—HIF-1α; placental growth factor—PIGF; transforming growth factor β—TGF-β; vascular endothelial growth factor—VEGF) at the mRNA and protein levels were assessed. It was found that every combined administration of a flavonoid and methyldopa in these cells induced a down-regulating effect on all tested factors, except PIGF, especially at the mRNA expression level. As hypertension generally raises TNF-α, IL-1β, IL-6, HIF-1α, TGF-β, and VEGF mRNA expression and/or protein levels, the results obtained in the studied model may provide a positive prognostic factor for such activity in vivo.
机译：该研究的目的是调查黄酮类化合物（Apigenin，Baicalin，Chrysin，槲皮素和Scutellarin）和甲基醇对在体外所选促炎和血管因子的表达中的综合作用，以预测其在妊娠高血压中的作用。该研究是在滋养细胞衍生的人核细胞细胞系和一次人脐静脉内皮细胞系上进行的。使用MTT试验测量选定浓度（20,40和100μmol）中化合物的细胞毒性，选择40μmol的浓度进行进一步分析。随后，它们对甲基二戊基对炎症（TNF-α; IL-1β; IL-6）和血管作用的表达的影响（TNF-α; IL-6）和血管作用（缺氧诱导因子1α-HIF-1α;胎盘生长因子 - 猪;转化生长评估MRNA和蛋白质水平的因子β-TGF-β;血管内皮生长因子-VEGF）。发现这些细胞中的黄酮类化合物和甲基甲戊基的每种组合给药诱导了对所有测试因子的下调效果，除仔猪外，尤其是mRNA表达水平。由于高血压通常提高TNF-α，IL-1β，IL-6，HIF-1α，TGF-β和VEGF mRNA表达和/或蛋白质水平，所得模型中获得的结果可以为这种活性提供阳性预后因素体内。

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