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首页> 美国卫生研究院文献>Molecules >RORα Regulates Odontoblastic Differentiation and Mediates the Pro-Odontogenic Effect of Melatonin on Dental Papilla Cells
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RORα Regulates Odontoblastic Differentiation and Mediates the Pro-Odontogenic Effect of Melatonin on Dental Papilla Cells

机译：RORα调节Odontobolas弹性分化并介导褪黑素对牙乳头细胞的促幼儿植物

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Dental papilla cells (DPCs), precursors of odontoblasts, are considered promising seed cells for tissue engineering. Emerging evidence suggests that melatonin promotes odontoblastic differentiation of DPCs and affects tooth development, although the precise mechanisms remain unknown. Retinoid acid receptor-related orphan receptor α (RORα) is a nuclear receptor for melatonin that plays a critical role in cell differentiation and embryonic development. This study aimed to explore the role of RORα in odontoblastic differentiation and determine whether melatonin exerts its pro-odontogenic effect via RORα. Herein, we observed that RORα was expressed in DPCs and was significantly increased during odontoblastic differentiation in vitro and in vivo. The overexpression of RORα upregulated the expression of odontogenic markers, alkaline phosphatase (ALP) activity and mineralized nodules formation (p < 0.05). In contrast, odontoblastic differentiation of DPCs was suppressed by RORα knockdown. Moreover, we found that melatonin elevated the expression of odontogenic markers, which was accompanied by the upregulation of RORα (p < 0.001). Utilising small interfering RNA, we further demonstrated that RORα inhibition attenuated melatonin-induced odontogenic gene expression, ALP activity and matrix mineralisation (p < 0.01). Collectively, these results provide the first evidence that RORα can promote odontoblastic differentiation of DPCs and mediate the pro-odontogenic effect of melatonin.
机译：牙科乳头细胞（DPC），牙卵细胞的前体被认为是组织工程的有前途的种子细胞。新兴的证据表明，褪黑素促进了DPC的Odontobolas弹性分化并影响牙齿发育，尽管确切的机制仍然未知。类视黄酸受体相关的孤儿α（RORα）是褪黑素的核受体，其在细胞分化和胚胎发育中起着关键作用。本研究旨在探讨RORα在Odontobolas弹性分化中的作用，并确定褪黑激素是否通过RORα施加其亲肠源性作用。在此，我们观察到RORα在DPC中表达，并且在体外和体内的Odontobolas弹性分化期间显着增加。 RORα的过表达上调了牙肠癌，碱性磷酸酶（ALP）活性和矿化结节形成的表达（P <0.05）。相反，通过RORα敲低抑制了DPC的Odontobolas弹性分化。此外，我们发现褪黑激素升高了异肠癌的表达，其伴随着RORα的上调（P <0.001）。利用小干扰RNA，我们进一步证明了RORα抑制减毒的褪黑激素诱导的牙肠病基因表达，ALP活性和基质矿化（P <0.01）。这些结果集体提供了第一种证据，即RORα可以促进DPC的异常分化并介导褪黑激素的亲渡育效果。

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