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首页> 美国卫生研究院文献>Plants >Recent Large-Scale Genotyping and Phenotyping of Plant Genetic Resources of Vegetatively Propagated Crops
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Recent Large-Scale Genotyping and Phenotyping of Plant Genetic Resources of Vegetatively Propagated Crops

机译：近大型基因分型和植物遗传资源植物遗传资源的植物遗传资源

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摘要

Several recent national and international projects have focused on large-scale genotyping of plant genetic resources in vegetatively propagated crops like fruit and berries, potatoes and woody ornamentals. The primary goal is usually to identify true-to-type plant material, detect possible synonyms, and investigate genetic diversity and relatedness among accessions. A secondary goal may be to create sustainable databases that can be utilized in research and breeding for several years ahead. Commonly applied DNA markers (like microsatellite DNA and SNPs) and next-generation sequencing each have their pros and cons for these purposes. Methods for large-scale phenotyping have lagged behind, which is unfortunate since many commercially important traits (yield, growth habit, storability, and disease resistance) are difficult to score. Nevertheless, the analysis of gene action and development of robust DNA markers depends on environmentally controlled screening of very large sets of plant material. Although more time-consuming, co-operative projects with broad-scale data collection are likely to produce more reliable results. In this review, we will describe some of the approaches taken in genotyping and/or phenotyping projects concerning a wide variety of vegetatively propagated crops.
机译：最近几个国家和国际项目的重点是植物遗传资源的大规模基因分型，植物繁殖的作物，如果实和浆果，土豆和木质饰品。主要目标通常是识别真正的植物材料，检测可能的同义词，并调查遗传多样性和遗传多样性和相关性。次要目标可能是创建可持续数据库，这些数据库可以在未来几年内使用研究和繁殖。常用的DNA标记（如微卫星DNA和SNP）和下一代测序，每个测序都具有它们的利弊。大规模表型的方法落后于后面，这是难以得分的许多商业重要性性状（产量，生长习性，沉积性和抗病性）。然而，对基因作用的分析和鲁棒DNA标记的发育取决于环境控制的非常大的植物材料的筛选。虽然具有宽尺寸数据收集的更耗时的合作项目可能会产生更可靠的结果。在本综述中，我们将描述关于各种植物繁殖作物的基因分型和/或表型项目中采取的一些方法。

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