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首页> 美国卫生研究院文献>Plants >Linking Tissue Damage to Hyperspectral Reflectance for Non-Invasive Monitoring of Apple Fruit in Orchards
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Linking Tissue Damage to Hyperspectral Reflectance for Non-Invasive Monitoring of Apple Fruit in Orchards

机译：将组织损伤与果园苹果果实的非侵入性监测联系起来的高光谱反射率

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摘要

Reflected light carries ample information about the biochemical composition, tissue architecture, and physiological condition of plants. Recent technical progress has paved the way for affordable imaging hyperspectrometers (IH) providing spatially resolved spectral information on plants on different levels, from individual plant organs to communities. The extraction of sensible information from hyperspectral images is difficult due to inherent complexity of plant tissue and canopy optics, especially when recorded under ambient sunlight. We report on the changes in hyperspectral reflectance accompanying the accumulation of anthocyanins in healthy apple (cultivars Ligol, Gala, Golden Delicious) fruits as well as in fruits affected by pigment breakdown during sunscald development and phytopathogen attacks. The measurements made outdoors with a snapshot IH were compared with traditional “point-type” reflectance measured with a spectrophotometer under controlled illumination conditions. The spectra captured by the IH were suitable for processing using the approaches previously developed for “point-type” apple fruit and leaf reflectance spectra. The validity of this approach was tested by constructing a novel index mBRI (modified browning reflectance index) for detection of tissue damages on the background of the anthocyanin absorption. The index was suggested in the form of mBRI = (R640−1 + R800−1) − R678−1. Difficulties of the interpretation of fruit hyperspectral reflectance images recorded in situ are discussed with possible implications for plant physiology and precision horticulture practices.
机译：反射光带有有关生化组成，组织结构和植物的生理状况的充分信息。最近的技术进步已经为实惠的成像高光谱计（IH）为不同水平的植物提供空间解决的光谱信息，从个体植物器官到社区。由于植物组织和冠层光学器件的固有复杂性，特别是在环境阳光下记录时，来自高光谱图像的显着的信息难以。我们报告了伴随着健康苹果（品种Ligol，Gala，Golden Delicious）水果以及在阳光发育和植物病理学发作期间受色素分解影响的水果中的花青素的蓄水率的变化。与快照IH进行的测量与在受控照明条件下用分光光度计测量的传统的“点型”反射率进行了比较。由IH捕获的光谱适用于使用先前为“点型”苹果果和叶反射谱发育的方法来处理。通过构建新的指数MBRI（改进的褐变反射率指数）来测试这种方法的有效性，用于检测花青素吸收的背景上的组织损伤。该指数以MBRI =（R640-1 + R800-1）的形式提出 - R678-1。讨论了植物生理学和精密园艺实践的可能影响，讨论了果实高光谱反射图像的果实的解释困难。

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