• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Plants >Computational Identification and Comparative Analysis of Conserved miRNAs and Their Putative Target Genes in the
【2h】

Computational Identification and Comparative Analysis of Conserved miRNAs and Their Putative Target Genes in the

机译：保守miRNA的计算鉴定与对比分析及其推定靶基因

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

MicroRNAs (miRNAs) are important factors for the post-transcriptional regulation of protein-coding genes in plants and animals. They are discovered either by sequencing small RNAs or computationally. We employed a sequence-homology-based computational approach to identify conserved miRNAs and their target genes in Persian (English) walnut, Juglans regia, and its North American wild relative, J. microcarpa. A total of 119 miRNA precursors (pre-miRNAs) were detected in the J. regia genome and 121 in the J. microcarpa genome and miRNA target genes were predicted and their functional annotations were performed in both genomes. In the J. regia genome, 325 different genes were targets; 87.08% were regulated by transcript cleavage and 12.92% by translation repression. In the J. microcarpa genome, 316 different genes were targets; 88.92% were regulated by transcript cleavage and 11.08% were regulated by translation repression. Totals of 1.3% and 2.0% of all resistance gene analogues (RGA) and 2.7% and 2.6% of all transcription factors (TFs) were regulated by miRNAs in the J. regia and J. microcarpa genomes, respectively. Juglans genomes evolved by a whole genome duplication (WGD) and consist of eight pairs of fractionated homoeologous chromosomes. Within each pair, the chromosome that has more genes with greater average transcription also harbors more pre-miRNAs and more target genes than its homoeologue. While only minor differences were detected in pre-miRNAs between the J. regia and J. microcarpa genomes, about one-third of the pre-miRNA loci were not conserved between homoeologous chromosome within each genome. Pre-miRNA and their corresponding target genes showed a tendency to be collocated within a subgenome.
机译：microRNA（miRNA）是植物和动物蛋白质编码基因转录调节的重要因素。通过测序小RNA或计算来发现它们。我们采用基于序列同源性的计算方法来识别Persian（英语）核桃，Juglans Regia及其北美野生相关，J.Microcarpa的保守miRNA及其目标基因。在J.Regia Genome和J.Mircarpa基因组中检测到总共119个miRNA前体（预麦芽染料），预测了MIRCARPA基因组和miRNA靶基因的121个，并且它们在两个基因组中进行了其功能注释。在J.Regia Genome中，325个不同的基因是靶标; 87.08％受转录裂解和翻译抑制的12.92％调节。在J.Microcarpa Genome中，316种不同的基因是靶标; 88.92％由转录物切割调节，11.08％通过翻译抑制调节。在J.Scia和J.Microcarpa Genomes中，MiRNA分别对所有抗性基因类似物（RGA）和2.7％和2.7％和2.6％的总转录因子（TFS）的总量分别受到近1.3％和2.7％和2.6％。 juglans基因组通过全基因组复制（WGD）演变，由八对分级的阳性染色体组成。在每对中，具有更多平均转录的基因的染色体还具有比其同源性更高的前麦芽糖和更多的靶基因。虽然在J.Regia和J.Microcarpa基因组之间仅在MIRNA之间检测到微细差异，但在每个基因组内的同源染色体之间没有保守额外三分之一的前三分之一。前miRNA及其相应的靶基因显示出在亚基组内脱离的趋势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号