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Metabolomic and transcriptomic signatures of prenatal excessive methionine support nature rather than nurture in schizophrenia pathogenesis

机译:产前过量蛋氨酸的代谢组和转录组特征而不是精神分裂症发病机制的培养

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摘要

a–c Maternal behaviors assessed in postpartum mice injected with SAL or MET during their last week of pregnancy. Box-whisker plots show median values and 25th/75th percentiles with minimum and maximum whiskers. a Pups retrieval latency by SAL and MET mothers, (nSAL = 5, nMET = 5), unpaired t-test, P > 0.05. b Pups retrieval duration of SAL and MET mice, (nSAL = 5, nMET = 5), unpaired t-test, P > 0.05. c The score of the nesting quality of SAL and MET mice, (nSAL = 5, nMET = 5), unpaired t-test, P > 0.05. d Diagram showing the design of cross-fostering experiment. e–h Behavioral tests assessed in adult male pups. e Distance mice travelled in 60 min of the locomotion assay (nSAL-SAL = 7, nMET-MET = 8, nSAL-MET = 9, nMET-SAL = 9), One-way ANOVA test (F = 4.8, P = 0.0058): *P < 0.05. Data are presented as means ± S.E.M. f Prepulse inhibition ratios against three prepulse stimulations in the PPI assay (nSAL-SAL = 11, nMET-MET = 13, nSAL-MET = 14, nMET-SAL = 13). One-way ANOVA followed by multiple comparisons test (F = 14.26, P < 0.0001), *P < 0.05, **P < 0.01. Box-whisker plots show median values and 25th/75th percentiles with minimum and maximum whiskers. g Percentage of the alternation choice mice made in the T-maze spontaneous assay (nSAL-SAL = 14, nMET-MET = 14, nSAL-MET = 14, nMET-SAL = 13). One-way ANOVA followed by Tukey post-test (F = 8.8, P = 0.0002), *P < 0.05, **P < 0.01, ***P < 0.001. Data are presented as means ± S.E.M. h Decision latency in the T-maze spontaneous assay. One-way ANOVA followed by Tukey post-test (F = 3.667, P > 0.05).
机译:在妊娠上一周内注射萨尔或满足的产后小鼠评估的A-C母体行为。 Box-Whisker Plots显示中位数和25/75百分位,最小和最大晶须。萨尔和遇到母亲的幼崽检索潜伏期,(NSAL = 5,NMET = 5),未配对T检验,P> 0.05。 B PUPS检索含SAL和Met MICE的持续时间,(NSAL = 5,NMET = 5),未配对T检验,P> 0.05。 c嵌套质量的分数和含铅小鼠,(NSAL = 5,NMET = 5),未配对T检验,P> 0.05。 D图显示了交叉培养实验的设计。成年雄性幼崽评估的E-H行为试验。 E距离小鼠在60分钟的运动测定中进行(NSAL-SAL = 7,NOME-MET = 8,NSAL-MET = 9,NET-SAL = 9),单向ANOVA测试(F = 4.8,P = 0.0058 ):* P <0.05。数据显示为平均值±S.E.M. F PPI测定中的三种预脉冲刺激的F预防抑制比(NSAL-SAL = 11,NMET-MET = 13,NMET-MET = 14,NMET-SAL = 13)。单向ANOVA,后跟多种比较测试(F = 14.26,P <0.0001),* P <0.05,** P <0.01。 Box-Whisker Plots显示中位数和25/75百分位,最小和最大晶须。在T-迷宫自发性测定中制备的交替选择小鼠的G百分比(NSAL-SAL = 14,NOME-MET = 14,NMER-MET = 14,NMET-SAL = 13)。单向ANOVA后跟Tukey后测试(F = 8.8,P = 0.0002),* P <0.05,** P <0.01,*** P <0.001。数据显示为平均值±S.E.M. H在T-Maze自发性测定中的决策延迟。单向Anova,后跟Tukey后测试(f = 3.667,p> 0.05)。

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