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首页> 美国卫生研究院文献>Journal of Animal Science >Influence of forage level and corn grain processing on whole-body urea kinetics and serosal-to-mucosal urea flux and expression of urea transporters and aquaporins in the ovine ruminal duodenal and cecal epithelia
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Influence of forage level and corn grain processing on whole-body urea kinetics and serosal-to-mucosal urea flux and expression of urea transporters and aquaporins in the ovine ruminal duodenal and cecal epithelia

机译：饲料水平和玉米籽粒加工对全身尿素动力学的影响以及血清瘤尿素尿素通量的血清瘤尿素和鼻腔十二指肠和颈蛋白上皮细胞

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The objectives were to determine the effects of forage level and grain processing on whole-body urea kinetics, N balance, serosal-to-mucosal urea flux (Jsm-urea), and messenger ribonucleic acid (mRNA) abundance of urea transporter-B (UT-B; SLC14a1) and aquaporins (AQP) in ovine ruminal, duodenal, and cecal epithelia. Thirty-two wether lambs were blocked by body weight into groups of four and assigned to one of four diets (n = 8) in a 2 × 2 factorial design. Dietary factors were forage level (30% [LF] vs. 70% [HF]) and corn grain processing (whole-shelled [WS] vs. steam-flaked [SF]). Four blocks of lambs (n = 4) were used to determine urea kinetics and N balance using 4-d [15N15N]-urea infusions with concurrent fecal and urine collections. Lambs were killed after 23 d of dietary adaptation. Ruminal, duodenal, and cecal epithelia were collected to determine Jsm-urea and mRNA abundance of UT-B and AQP. Lambs fed LF had greater intakes of dry matter (DMI; 1.20 vs. 0.86 kg/d) and N (NI; 20.1 vs. 15.0 g/d) than those fed HF (P < 0.01). Lambs fed SF had greater DMI (1.20 vs. 0.86 kg/d) and NI (20.6 vs. 14.5 g/d) than those fed WS (P < 0.01). As a percentage of NI, total N excretion was greater in lambs fed HF compared with those fed LF (103% vs. 63.0%; P < 0.01) and was also greater in lambs fed WS compared with those fed SF (93.6% vs. 72.1%; P = 0.02). Retained N (% of NI) was greater in lambs fed LF compared with those fed HF (37.0% vs. −2.55%; P < 0.01). Lambs fed SF had a greater (P = 0.02) retained N (% of NI; 28.0% vs. 6.50%) compared with those fed WS. Endogenous urea production (UER) tended (P = 0.09) to be greater in lambs fed HF compared with those fed LF. As a proportion of UER, lambs fed HF had a greater urinary urea-N loss (0.38 vs. 0.22) and lower urea-N transferred to the gastrointestinal tract (GIT; 0.62 vs. 0.78) or urea-N used for anabolism (as a proportion of urea-N transferred to the GIT; 0.12 vs. 0.26) compared with lambs fed LF (P < 0.01). Ruminal Jsm-urea was unaffected by diet. Duodenal Jsm-urea was greater (P < 0.01) in lambs fed HF compared with LF (77.5 vs. 57.2 nmol/[cm2 × h]). Lambs fed LF had greater (P = 0.03) mRNA expression of AQP3 in ruminal epithelia and tended (P = 0.06) to have greater mRNA expression of AQP3 in duodenal epithelia compared with lambs fed HF. Expression of UT-B mRNA was unaffected by diet. Our results showed that feeding more ruminally available energy improved N utilization, partly through a greater proportion of UER being transferred to the GIT and being used for anabolic purposes.
机译：目的是确定饲料水平和粮食加工对全身尿素动力学，N平衡，浆膜对粘膜尿素通量（JSM-utea）和信使核糖核酸（mRNA）丰富的尿素转运蛋白-B（ UT-B; SLC14A1）和Aquaporins（AQP）在绵瘤，十二指肠和宫颈上皮细胞中。将三十两种衍生体重堵塞成四组，并以2×2因子设计分配到四种饮食（n = 8）中的一种。膳食因子是饲料水平（30％[LF] vs. 70％[HF]）和玉米粒加工（全壳[WS]与蒸汽碎片[SF]）。使用4-D [15N15N] - 用同时粪便和尿液收集来确定尿素动力学和N平衡的四个羊羔（n = 4）。在23 d膳食适应后，羔羊被杀死。收集瘤胃，十二指肠和宫颈上皮，以确定JSM-UREA和MRNA丰富的UT-B和AQP。羔羊饲喂LF的干物质进气（DMI; 1.20 vs.0.86 kg / d）和n（ni; 20.1 vs.15.0g / d）的进气量比喂养hf（p <0.01）。羔羊喂养SF具有较大的DMI（1.20 vs.0.86 kg / d）和Ni（20.6 vs.14.5g / d），而不是喂养Ws（P <0.01）。作为Ni的百分比，与喂养LF（103％与63.0％; p <0.01）相比，乳液中总N排出量更大，与乳头Ws的羊绒相比，乳液中的含量也更大（93.6％对比。 72.1％; p = 0.02）。与喂养HF的那些（37.0％对-2.55％; P <0.01）相比，在乳腺饲料LF中保留N（Ni％）更大。与喂养WS的人相比，羔羊喂养SF具有更大的（P = 0.02），保留N（Ni的百分比，效率为28.0％，vs.6.50％）。与喂养LF相比，内源性尿素产生（uer）倾向于（p = 0.09）达到HF的羊羔。作为uer的比例，乳液进料Hf具有更大的尿尿素尿素损失（0.38 vs.022）和转移到胃肠道（git; 0.62 vs.0.78）或用于合成代谢的尿素-N的下尿素-N（如与乳绒LF相比，将0.12与0.26转移到Git的尿素-N的比例（P <0.01）。瘤胃JSM-UREA不受饮食影响。 Duodenal JSM-UREA在乳头FED HF中更大（P <0.01），与LF（77.5 Vs.57.2 Nmol / [Cm 2×H]）进行比较。乳液喂养的LF具有更大的（p = 0.03）AQP3在瘤胃上皮细胞中的mRNA表达，与FED HF相比，在十二指肠上皮细胞中具有更大的MRNA表达AQP3。 UT-B mRNA的表达不受饮食的影响。我们的结果表明，部分通过将更大比例的UER转移到Git并用于代谢目的，部分地喂养更酷测的能量提高了N利用率。

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