• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Animal Science >PSVI-35 The combined effects of early-socialization of piglets and neonatal enriched environment on intestinal gene expression and fecal community structure
【2h】

PSVI-35 The combined effects of early-socialization of piglets and neonatal enriched environment on intestinal gene expression and fecal community structure

机译：PSVI-35仔猪早期社会化对肠道基因表达和粪便群落结构的综合影响

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The management of the litters through practices of early-socialization and environmental enrichment has been shown to improve piglet’s adaptation at weaning, reducing stress response. We hypothesized that changes in the neonatal environment of piglets could also modulate the maturation of intestinal microbiota and gene expression at weaning. In a commercial farm, 14 maternity sows and their litters were allotted to two treatments: a control treatment (commercial conditions, CTR) and an enriched treatment (ENR) in which piglets from two litters were mixed 14 days post-partum by removing fences. Moreover, the farrowing pen was fitted with hanging objects. Piglets were mixed after weaning (d28) according to their body size, as in commercial practice, but keeping experimental groups. Faecal and jejunum samples were collected from 7 piglets/treatment before (d26) and after (d31) weaning. Faecal microbiome was analyzed by sequencing the 16S RNA gene (Illumina MiSeq®) and OpenArray® technology was used for gene expression analysis. No significant changes promoted by treatments were found in microbiota structure during lactation. However, dissimilarities were observed after weaning (Penvfit = 0.04) although we were not able to detect significant changes in particular taxa. Weaning had an evident impact in the microbiota structure with increases in α-diversity and a clear decrease in Lactobacillaceae family. Regarding intestinal gene expression, a higher expression of the TLR2 gene was registered in CTR piglets after weaning (P = 0.03). The weaning process itself was associated with changes in the expression of numerous genes related to barrier function, digestive enzymes and nutrient transport. Results confirm that early socialization of piglets and an enriched neonatal environment during lactation, can have an impact on the maturation of the intestinal microbiota after weaning. These effects could be mediated by a differential stress response and changes in the cross-talk between the host and the intestinal microbiota.
机译：通过早期社会化和环境富集的做法，潜水者的管理已被证明可以改善仔猪对断奶的适应，降低压力反应。我们假设仔猪的新生儿环境的变化也可以调节肠道微生物群和断奶中的基因表达的成熟。在商业农场中，分配了14个产妇母猪及其窝点：通过去除围栏，对照治疗（商业条件，CTR）和富集的治疗（ENR），其中两次窝的仔猪混合14天。此外，击球笔配有悬挂物体。根据其体尺寸（D28）在商业实践中，仔猪混合仔猪，但保持实验组。在（D26）之前和（D31）断奶后，从7颗仔猪/治疗中收集粪便和JEUNUM样品。通过测序16S RNA基因（IlluminaMiseq®）和OpenArray®技术用于基因表达分析来分析粪便微生物组。在泌乳期间，在微生物群结构中发现了治疗促进的重大变化。然而，断奶后观察到异化（PENVFIT = 0.04），尽管我们无法检测特定的分类群的重大变化。断奶对微生物群结构具有明显的影响，随着α-多样性的增加，乳酸杆菌家族的含量明显减少。关于肠道基因表达，在断奶后在CTR仔猪中登记TLR2基因的更高表达（P = 0.03）。断奶过程本身与与障碍功能，消化酶和营养转运相关的许多基因表达的变化有关。结果证实，仔猪的早期社会化和富集的新生儿环境，在断奶后会对肠道微生物群的成熟产生影响。这些效果可以通过差分应力响应和宿主和肠道微生物群系之间的串扰的变化来介导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号