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首页> 美国卫生研究院文献>Pathogens >Efficient Transovarial Transmission of Babesia Spp. in Rhipicephalus microplus Ticks Fed on Water Buffalo (Bubalus bubalis)
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Efficient Transovarial Transmission of Babesia Spp. in Rhipicephalus microplus Ticks Fed on Water Buffalo (Bubalus bubalis)

机译：Babesia SPP的高效跨动力传输。在Rapicephalus microplus microplus inft在水牛喂食（Bubalus bubalis）

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Water buffaloes can be infected by tick-borne pathogens (TBPs) in endemic areas where cattle and buffalo coexist. Among TBPs affecting buffaloes is the Apicomplexan hemoparasites Babesia bovis and B. bigemina, transmitted by Rhipicephalus microplus ticks. However, little empirical evidence exists on whether buffalo can support TBPs’ infection and transmission. A cohort study was designed to measure the infestation levels of R. microplus in buffaloes as well as the ability of buffalo-fed ticks to transmit B. bovis and B. bigemina to their offspring. Tick infestation of different life stages was quantified in cattle and buffalo kept in field conditions in western Cuba. Engorged adult female ticks were allowed to lay eggs in controlled conditions of humidity and temperature, and reproductive parameters were measured and analyzed. Hosts and tick larvae were tested for the presence of Babesia spp. using species-specific qPCR assays. Tick infestation was not observed in adult buffaloes. However, buffalo and cattle calves were equally infested, although the larval survival rate was higher in cattle calves than in buffalo calves. All larval pools (31) obtained from the adult female ticks were positive for B. bovis, whereas only 68% (21/31) was positive for B. bigemina. Among the 10 larval pools negative for B. bigemina, three proceeded from adult females fed on Babesia-negative buffaloes. The other seven pools were from Babesia-positive animals, three from cattle and four from buffalo calves. Babesia infection levels in tick larvae, quantified by qPCR, were similar in female ticks fed on buffalo and bovine calves. We conclude that water buffalo can sustain tick vector populations and support Babesia infection in levels high enough as to be infective for ticks. Our results also validated the hypothesis that adult female ticks fed on buffalo can transmit the pathogens B. bovis and B. bigemina to their offspring. Nevertheless, further laboratory studies are needed to address the question of whether the transovarial transmission of Babesia occurs in the following settings: (1) When adult females are infected previous to the feeding on the buffalo or/and (2) when the adult females acquire the infection while feeding on the buffalo.
机译：水牛在牛和水牛共存的地方区域中的蜱传播病原体（Tbps）可以感染。在影响水牛的Tbps中，ApiCoMplexan Hemoparasites Babesia Bovis和B.Bigemina，由Rapicephalus microplus蜱传播。但是，水牛是否能够支持Tbps的感染和传输，存在很少的经验证据。队列研究被设计用来测量R. microplus的侵染水平水牛以及水牛喂养蜱发送B.杆菌的能力和B. bigemina遗传给后代。在牛和水牛的粪便和水牛中量化了不同寿命的蜱灭绝，在古巴西部的现场条件下。允许收集的成年女性蜱虫在受控湿度和温度的控制条件下产卵，并测量并分析生殖参数。在Babesia SPP的存在下测试了宿主和蜱幼虫。使用特异性QPCR测定。在成人的水牛中没有观察到蜱灭绝。然而，水牛和牛犊同样侵染，尽管牛犊的幼虫存活率比水牛犊在牛犊较高。从成年女性蜱虫获得的所有幼虫池（31）都是B. Bovis的阳性，而只有68％（21/31）对于B.Bigemina是阳性的。在B. Bigemina的10个幼虫池中，三个从喂养Babesia阴性水牛喂养的成年女性进行了三个。其他七个游泳池来自Babesia阳性动物，三个来自牛和来自水牛小牛的四个。蜱幼虫的Babesia感染水平，通过QPCR量化，在喂食水牛和牛犊的女性蜱中类似。我们得出结论，水水牛可以维持蜱虫群，并支持足够高的水平的Babesia感染，以对蜱感染。我们的结果还经过验证了在水牛喂养的成年女性蜱虫可以将病原体B. Bovis和B. Bigemina发送给他们的后代。然而，需要进一步的实验室研究来解决在以下设置中发生Babesia的转发递送：（1）当成年女性被感染到Buffalo或/和（2）之前被感染成人女性收购时在水牛上喂养的感染。

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