• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Toxicon: X >Partial proteomic analysis of brown widow spider (Latrodectus geometricus) venom to determine the biological activities
【2h】

Partial proteomic analysis of brown widow spider (Latrodectus geometricus) venom to determine the biological activities

机译：棕色寡妇蜘蛛（Latrodectus Geometricus）毒液的部分蛋白质组学分析确定生物学活性

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Spiders use their venom for defence and to capture prey. These venoms contain a cocktail of biologically active compounds that display several different biological activities, such as large molecules and small molecules including peptides, proteins/enzymes, and other components. Thus, venom constituents have attracted the attention of biochemists and pharmacologists over the years. The brown widow spider (Latrodectus geometricus) is a venomous spider found worldwide, including in Thailand. This spider causes human injuries, and the venom has many potential applications. In this study, we investigated the complexity and pharmacology of brown widow spider venom. Spider crude venom was investigated using partial proteome techniques and enzymatic activity, toxicity, and antibacterial activity assessments. We found that crude venom displayed a wide range of molecular masses from 19 to over 97 kDa, with molecular masses of 66 kDa intensely stained. Peptides and proteins were identified by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), which showed that the crude venom contained a variety of substances, including latrotoxins, apolipophorins, hemocyanins, chitinases, arginine kinase, allergen antigen 5-like protein, astacin-like metalloproteases, and serine proteases. High hyaluronidase activity was observed based on the turbidimetric method. The venom presented toxicity in crickets (PD50 = 0.73 ± 0.10 μg/g body weight), and substantial envenomation symptoms, such as slow-motion movement, paralysis, and even death, were noted. Moreover, this venom exhibited potential antibacterial activity against the gram-positive Bacillus subtilis but not the gram-negative Pseudomonas aeruginosa. Spider venom contains numerous molecules with biological activity, such as latrotoxins, which affect insects, and enzymes. In addition to latrotoxins, certain enzymes in venom are hypothesized to exhibit toxicity and antimicrobial activity. This study provides important information for the further development of natural compounds or insecticidal toxins.
机译：蜘蛛使用他们的毒液来防御并捕捉猎物。这些毒液含有一种生物活性化合物的鸡尾酒，其显示出几种不同的生物活性，例如大分子和小分子，包括肽，蛋白质/酶和其他组分。因此，毒液成分在多年来引起了生物化学派和药剂学家的注意。棕色寡妇蜘蛛（Latrodectus geometricle）是全球发现的毒蜘蛛，包括在泰国。这种蜘蛛会引起人类伤害，毒液具有许多潜在的应用。在这项研究中，我们研究了棕色寡妇蜘蛛毒液的复杂性和药理学。使用部分蛋白质组技术和酶活性，毒性和抗菌活性评估来研究蜘蛛粗毒液。我们发现原油毒液从19至超过97kDa展示了广泛的分子量，其中分子量为66kDa强烈染色。通过液相色谱 - 质谱/质谱/质谱（LC-MS / MS）鉴定肽和蛋白质，表明粗毒液含有各种物质，包括拉滴毒素，脂素素，血红蛋白，几丁质酶，精氨酸激酶，过敏原抗原5-与蛋白质一样，斯卡蛋白样金属蛋白酶和丝氨酸蛋白酶。基于浊度法观察高透明质酸酶活性。毒液在蟋蟀中呈现毒性（PD50 = 0.73±0.10μg/ g体重），并注意到慢动作运动，瘫痪甚至死亡等大量的envenomation症状，如慢动作运动，瘫痪甚至死亡。此外，这种毒液表现出对革兰氏阳性枯草芽孢杆菌的潜在抗菌活性，但不是革兰氏阴性假单胞菌铜绿假单胞菌。蜘蛛毒液含有许多具有生物活性的分子，例如影响昆虫的Latrotoxins和酶。除了拉伸毒素外，毒液中的某些酶被假设为表现出毒性和抗微生物活性。本研究提供了进一步发展天然化合物或杀虫毒素的重要信息。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号