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首页> 美国卫生研究院文献>Biochemistry and Biophysics Reports >Increased benzoxazinoid (Bx) levels in wheat seedlings via jasmonic acid treatment and etiolation and their effects on Bx genes including Bx6
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Increased benzoxazinoid (Bx) levels in wheat seedlings via jasmonic acid treatment and etiolation and their effects on Bx genes including Bx6

机译：通过Jasmonic酸处理和黄光酸盐和物质化合物在小麦幼苗中增加苯并恶嗪（Bx）水平及其对Bx基因的影响包括BX6

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Wheat accumulates benzoxazinoid (Bx) as a defensive compound. While Bx occurs at high concentrations, particularly in the early growth stages, its mechanism of regulation remains unclear. In the present study, we first examined the effects of several plant hormones on Bx concentrations in wheat seedlings. Among the compounds tested, jasmonate (JA) elevated the concentrations of DIMBOA-Glc (2-β-D-glucoside of 2,4-dihydroxy-7-methoy-1,4-benzoxazin-3-one), the primary Bx species in intact wheat seedlings, without a significant increase in HDMBOA-Glc (4-O-methyl-DIMBOA-Glc), which is known to be upregulated by stresses. In addition, growing the plants in the dark increased DIMBOA-Glc levels. Quantification of the Bx-biosynthetic genes showed that TaBx8 (UDP-Glc:Bx glucosyltrasferase) was influenced by neither JA nor etiolation, indicating that TaBx8 is under the regulation mechanism distinct from the mechanisms influencing the others. In addition, none of the other gene expression patterns exhibited considerable correlation with DIMBOA-Glc accumulation. Since there was no correlation between transcript levels of the genes involved in Bx biosynthesis and Bx accumulation, other factors may control the levels of Bx in wheat. In the course of gene analyses, we isolated TaBx6, one of the last two genes that had not been identified in wheat in the DIMBOA-Glc biosynthetic pathway. All the four TaBx6 genes cloned in the present study were expressed in Escherichia coli and characterized their activity.
机译：小麦将苯并恶嗪（BX）累积为防御化合物。虽然BX以高浓度发生，但特别是在早期生长阶段，其调节机制仍然不清楚。在本研究中，我们首先检查了几种植物激素对小麦幼苗浓度的影响。在测试的化合物中，茉莉酸酯（JA）升高了DiMboa-glc的浓度（2,4-二羟基-7-甲基-1,4-苯并恶化-3-一）的浓度（2-β-D-葡萄糖苷-1,4-苯并恶化-3-一）在完整的小麦幼苗中，没有显着增加HDMBOA-glc（4-甲基-iMboa-glc），已知通过应力上调。此外，在黑暗增加的DiMboa-glc水平上生长植物。 Bx-Biosynthetic基因的定量表明，Tabx8（UDP-glc：Bx葡糖基转移酶）既不是JA也不是金黄糖苷酸化，表明突出机构在不同影响其他机制的调节机制下。另外，没有其他基因表达模式与DiMboa-glc积累表现出相当大的相关性。由于涉及BX生物合成和Bx积累的基因的转录物水平之间没有相关性，因此其他因素可以控制小麦中Bx的水平。在基因分析过程中，我们隔离突变曲线曲线，在DiMba-glc生物合成途径中尚未鉴定在小麦中的最后两种基因之一。本研究中克隆的所有四个舌头基因在大肠杆菌中表达并表征了它们的活性。

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