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Evaluation of graded levels of Bacillus coagulans GBI-30 6086 on apparent nutrient digestibility stool quality and intestinal health indicators in healthy adult dogs

机译:评估芽孢杆菌凝硫酸盐糖尿病GBI-306086对健康成年犬表观营养消化率粪便质量和肠道健康指标的评分水平

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摘要

Bacillus coagulans GBI-30, 6086 is a commercially available spore-forming non-toxigenic microorganism approved for use in dog foods with high resiliency to stresses associated with commercial manufacturing. The objectives of this research were to examine the effect of B. coagulans on stool quality, nutrient digestibility, and intestinal health markers in healthy adult dogs. Extruded diets containing graded levels of B. coagulans applied either to the base ration before extrusion or to the exterior of the kibble as a topical coating after extrusion were randomly assigned to 10 individually housed adult beagle dogs (7 castrated males and 3 spayed females) of similar age (5.75 ± 0.23 yr) and body weight (12.3 ± 1.5 kg). The study was designed as a 5 × 5 replicated Latin square with 16-d adaptation followed by 5-d total fecal collection for each period. Five dietary treatments were formulated to deliver a dose of 0-, 6-, 7-, 8-, and 9-log10 colony-forming units (CFU) per dog per day for the control (CON), extruded B. coagulans (PEX), and low, moderate, and high B. coagulans coating levels (PCL, PCM, and PCH), respectively. Food-grade TiO2 was added to all diets at a level of 0.4% to serve as an indigestible dietary marker for digestibility calculations. Data were analyzed using a mixed model through SAS (version 9.4, SAS Institute, Inc., Cary, NC) with treatment as a fixed effect and room (i.e., replicate), period, and dog(room) as random effects. Apparent total tract digestibility of organic matter, crude protein, crude fat, and gross energy calculated by the marker method were numerically greatest for dogs fed the 9-log10 dose treatment with increases (P < 0.05) observed in gross energy and organic matter digestibility compared with the negative control. No significant differences were observed in food intake, stool quality, fecal pH, fecal ammonia, fecal short-chain fatty acids, or branched-chain fatty acids for the extruded B. coagulans treatment (PEX) or the coated B. coagulans treatments (PCL, PCM, and PCH) compared with CON. These results suggest that B. coagulans has a favorable impact on nutrient digestibility and no apparent adverse effects when added to extruded diets at a daily intake level of up to 9-log10 CFU in healthy adult dogs.
机译:芽孢杆菌凝磺酸甘油甘油GBI-30,6086是一种商业上可获得的孢子形成的非毒性微生物,用于狗食,其具有高弹性与与商业制造相关的胁迫。该研究的目标是检查B.Coγsans对健康成年犬粪便质量,营养消化率和肠道健康标志的影响。含有B的挤出饮食B.凝结在挤出前施用前的凝固素的凝固水平为基础比赛,作为浇铸后的局部涂层被随机分配给10个单独容纳的成人比格犬(7个阉割的男性和3个穿过女性)类似年龄(5.75±0.23 YR)和体重(12.3±1.5千克)。该研究设计为5×5复制的拉丁广场,具有16-D适应,然后为每个时段进行5-D总粪便收集。配制五种膳食处理,每天为每只狗提供每只狗的0-,6-,7-,8-和9-log10和9-log10的成分单元(CO),挤出的B.凝血蛋白(PEX ),低,中等和高B.凝胶蛋白涂层水平(PCL,PCM和PCH)。将食物级TiO 2加入到所有饮食中,以0.4%的水平,用作可消化性计算的难民膳食标志物。通过SAS(版本9.4,SAS Institute,Inc.,Cary,NC)使用混合模型进行分析数据,作为固定效果和房间(即,复制),期间和狗(房间)作为随机效果。有机物质的表观总易消化性,粗蛋白质,粗脂肪和通过标记方法计算的总能量为喂养9-log10剂量治疗的数量最大,随着总能量和有机物质消化率观察到的增加(p <0.05)有了负面控制。在食品摄入量,粪便质量,粪便pH,粪便氨,粪便短链脂肪酸或支链脂肪酸中没有观察到显着差异,用于挤出的B.凝壳治疗(PEX)或涂覆的B.凝血蛋白处理(PCL与孔相比,PCM和PCH)。这些结果表明B.凝固素对营养消化率有利影响,并且当在健康成年犬的每日摄入量的每日摄入水平时添加到挤出的饮食中,没有明显的不良反应。

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