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首页> 美国卫生研究院文献>Molecules >Essential Oils of New Lippia alba Genotypes Analyzed by Flow-Modulated Comprehensive Two-Dimensional Gas Chromatography (GC×GC) and Chemometric Analysis
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Essential Oils of New Lippia alba Genotypes Analyzed by Flow-Modulated Comprehensive Two-Dimensional Gas Chromatography (GC×GC) and Chemometric Analysis

机译：通过流量综合的综合二维气相色谱（GC×GC）和化学计量分析分析新的Lippia Alba基因型的精油和化学计量分析

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摘要

Lippia alba (Mill.) N. E. Br. (Verbenaceae) is an aromatic shrub whose essential oils have stood out as a promising source for application in several industrial fields. In this study, the essential oils chemical characterization of eight new L. alba genotypes was performed. The selected materials were collected from the Active Germplasm Bank of the Agronomic Institute and the essential oils were extracted by hydrodistillation. Flow-modulated comprehensive two-dimensional gas chromatography coupled to mass spectrometry (GC×GC-MS) was employed for chemical characterization and evaluation of possible co-eluted compounds. In addition, the chemical analyses were submitted to multivariate statistical analyses. From this investigation, 73 metabolites were identified in the essential oils of the genotypes, from which α-pinene, β-myrcene, 1,8-cineole, linalool, neral, geranial, and caryophyllene oxide were the most abundant compounds among the accessions. This is the first report disclosing α-pinene in higher amounts in L. alba (19.69%). In addition, sabinene, trans-verbenol, myrtenol, (E)-caryophyllene, α-guaiene, germacrene D, and α-bulnesene were also found in relevant quantities in some of the genotypes, and myrtenal and myrtenol could be well separated through the second dimension. Such results contributed to the understanding of the chemical composition of those new genotypes, being important to drive a future industrial applicability and studies in genetic breeding.
机译：Lippia Alba（磨机。）N.E.Br。 （verbenaceae）是一种芳香的灌木，其精油在几个工业领域的应用源代理。在这项研究中，进行了八种新型L. Alba基因型的精油化学表征。从农艺学院的活性种质群中收集所选材料，并通过氢化物提取精油。用于质谱（GC×GC-MS）的流量调节的综合二维气相色谱法用于化学表征和对可能的共洗脱化合物的评价。此外，化学分析提交给多元统计分析。从该研究中，在基因型的精油中鉴定了73种代谢物，其中α-拼蛋烯，β-氨基烯，1,8-鳞状，烯丙，颈醛，颅内，和亚氰基氧化物是载体中最丰富的化合物。这是第一个在L. Alba（19.69％）中更高量的α-pinene的报告。此外，在一些基因型中的相关数量中也发现了SabineNe，Trans-vermenol，Myrtenol，（E） - 甲基丙烯，α-胍烯，生殖器酮和α-硼酮，并且MyRtenal和Myrtenol可以通过以下方式分开很好地分开第二维。这种结果有助于了解这些新基因型的化学成分，这是推动未来的工业实用性和遗传育种研究的重要性。

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