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No Evidence for Seed Transmission of Tomato Yellow Leaf Curl Sardinia Virus in Tomato

机译：在番茄中没有番茄黄叶卷曲糖粉病毒种子传播的证据

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Seed transmission is an important factor in the epidemiology of plant pathogens. Geminiviruses are serious pests spread in tropical and subtropical regions. They are transmitted by hemipteran insects, but a few cases of transmission through seeds were recently reported. Here, we investigated the tomato seed transmissibility of the begomovirus tomato yellow leaf curl Sardinia virus (TYLCSV), one of the agents inducing the tomato yellow leaf curl disease, heavily affecting tomato crops in the Mediterranean area. None of the 180 seedlings originating from TYLCSV-infected plants showed any phenotypic alteration typical of virus infection. Moreover, whole viral genomic molecules could not be detected in their cotyledons and true leaves, neither by membrane hybridization nor by rolling-circle amplification followed by PCR, indicating that TYLCSV is not a seed-transmissible pathogen for tomato. Examining the localization of TYLCSV DNA in progenitor plants, we detected the virus genome by PCR in all vegetative and reproductive tissues, but viral genomic and replicative forms were found only in leaves, flowers and fruit flesh, not in seeds and embryos. Closer investigations allowed us to discover for the first time that these embryos were superficially contaminated by TYLCSV DNA but whole genomic molecules were not detectable. Therefore, the inability of TYLCSV genomic molecules to colonize tomato embryos during infection justifies the lack of seed transmissibility observed in this host.
机译：种子传输是在植物病原体流行病学的一个重要因素。双生病毒是在热带和亚热带地区严重的害虫蔓延。它们是由半翅目昆虫传播，而是通过种子传播的病例数最近的报道。在这里，我们调查了双生番茄黄化曲叶病毒撒丁岛（TYLCSV），代理人诱导番茄黄化曲叶病之一的番茄种子传递，严重影响番茄地中海地区的作物。从TYLCSV感染的植株180个苗始发均未发现任何表型变化的典型病毒感染。此外，整个病毒基因组的分子不能在它们的子叶和真叶检测，既不由膜杂交也不通过滚环扩增，随后通过PCR，表明TYLCSV不是番茄种子传播的病原体。检查TYLCSV DNA的祖先植物的本地化，我们发现通过PCR病毒基因组中的所有营养生长和生殖组织，但仅见于叶，花，果实肉厚，没有种子和胚胎病毒基因组复制和形式。建立更紧密的调查让我们发现，第一次，这些胚胎表面上由TYLCSV DNA，但全基因组分子污染检测不到。因此，基因组TYLCSV分子的不能在感染证明在此宿主中观察到的缺乏种子传递的定殖番茄胚胎。

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