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首页> 美国卫生研究院文献>Bosnian Journal of Basic Medical Sciences >Dishevelled family proteins (DVL1-3) expression in IUGR placentas
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Dishevelled family proteins (DVL1-3) expression in IUGR placentas

机译：IUGR胎盘中饰品饰品的家庭蛋白质（DVL1-3）表达

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Dishevelled family proteins (DVL1, DVL2, and DVL3) are cytoplasmic proteins that are involved in canonical and non-canonical Wnt signaling pathway during embryonic development. The role of DVL proteins in the placental tissue remains mostly unknown. In the current study, we explored the role of Dishevelled proteins in naturally invasive tissue, trophoblast. Formalin-fixed paraffin-embedded samples of 15 term placentas from physiologic term pregnancies and 15 term placentas from pregnancies complicated with intrauterine growth restrictions (IUGR) were used for the study. Expression levels of mRNA for DVL1, DVL2, and DVL3 in placentas were analyzed by quantitative real-time PCR (qRT-PCR). DVL1, DVL2, and DVL3 protein expression were semi-quantitatively analyzed using immunohistochemistry. The expression of DVL3 protein was significantly higher in trophoblasts and endothelial cells in placental villi from IUGR pregnancies compared with the control group of term placentas, while DVL2 protein expression was significantly higher in trophoblasts in placental villi from IUGR pregnancies compared with normal term placentas. The observed differences at protein levels between normal and IUGR placentas were not confirmed at the mRNA levels of DVL genes. Our data indicate the active involvement of DVL proteins in IUGR-related placentas. No significant changes were observed in DVL mRNA levels between the two groups of placentas. Further studies are required to explore the clinical relevance of these observations.
机译：蓬乱蛋白家族（DVL1，DVL2，和DVL3）是被胚胎发育过程中涉及的经典和非经典Wnt信号转导通路的细胞质蛋白。 DVL蛋白在胎盘组织中的作用仍然是未知的大部分。在目前的研究中，我们探讨蓬乱蛋白的自然侵入组织，滋养作用。福尔马林固定的从生理足月妊娠15个足月胎盘并从与宫内生长限制（IUGR）并发妊娠15个足月胎盘石蜡包埋的样品用于这项研究。对于DVL1，DVL2和DVL3胎盘mRNA表达水平通过定量实时PCR（定量RT-PCR）分析。 DVL1，DVL2，和DVL3蛋白表达的半定量免疫组织化学使用分析。 DVL3蛋白的表达在滋养层细胞和内皮细胞在胎盘绒毛显著更高从IUGR妊娠与足月胎盘的对照组比较，而DVL2蛋白表达显著高于滋养细胞在胎盘绒毛从IUGR妊娠与正常足月胎盘进行比较。在正常和IUGR胎盘之间蛋白水平观察到的差异在DVL基因的mRNA水平没有得到证实。我们的数据表明DVL蛋白在宫内发育迟缓，胎盘有关的积极参与。无显著变化两组胎盘之间DVL mRNA水平观察。需要进一步研究探讨这些观察结果的临床意义。

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