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Novel Protozoans in Austria Revealed through the Use of Dogs as Sentinels for Ticks and Tick-Borne Pathogens

机译:奥地利的新型原生动物通过使用狗作为哨兵的哨兵透露蜱和蜱传播病原体

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摘要

We previously isolated and cultivated the novel Rickettsia raoultii strain Jongejan. This prompted us to ask whether this strain is unique or more widely present in Austria. To assess this issue, we retrospectively screened ticks collected from dogs in 2008. Of these collected ticks, we randomly selected 75 (47 females and 28 males) Dermacentor reticulatus, 44 (21 females, 7 males, and 16 nymphs) Haemaphysalis concinna, and 55 (52 females and 3 males) ticks of the Ixodes ricinus complex. Subsequently, these ticks were individually screened for the presence of tick-borne pathogens using the reverse line blot hybridization assay. In our current study, we detected DNA from the following microbes in D. reticulatus: Anaplasma phagocytophilum, Borrelia lusitaniae, Borrelia spielmanii, Borrelia valaisiana, and R. raoultii, all of which were R. raoultii strain Jongejan. In H. concinna, we found DNA of a Babesia sp., Rickettsia helvetica, and an organism closely related to Theileria capreoli. Lastly, I. ricinus was positive for Anaplasma phagocytophilum, Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii/Borrelia bavariensis, B. lusitaniae, B. spielmanii, B. valaisiana, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, Rickettsia monacensis, and Theileria (Babesia) microti DNA. The detection of DNA of the Babesia sp. and an organism closely related to Theileria capreoli, both found in H. concinna ticks, is novel for Austria.
机译:我们以前分离和培养的新立克次氏体raoultii应变Jongejan。这促使我们要问这个菌株是否是唯一的或在奥地利更广泛存在。为了评估这个问题,我们回顾性筛选在2008年这些收集蜱从狗收集蜱,我们随机选择的75(47名女性和28名男性)革蜱网纹,44(21位女性,男性7例,和16个若虫)嗜群血蜱,和55(52名女性和3个男性)蜱硬蜱的蓖麻复杂。随后,这些蜱单独筛选的使用反向线杂交法蜱传病原体的存在。在我们的研究中,我们检测到的DNA从D.网纹以下微生物:无形体吞噬细胞,螺旋体葡萄牙念珠菌,疏螺旋体spielmanii,伯氏疏valaisiana和R. raoultii,所有这些都是R. raoultii应变Jongejan。在H.水蚤,我们发现了一个巴贝斯虫菌的DNA,立克次氏体黑体,以及密切相关的泰勒capreoli有机体。最后,一种蓖麻吞噬细胞无形体是积极的,螺旋体afzelii,莱姆病螺旋体狭义,螺旋体garinii /雷伯氏bavariensis,B.葡萄牙念珠菌,B. spielmanii,B. valaisiana,暂定Neoehrlichia mikurensis,立克次体黑体,立克次氏体monacensis和泰勒(巴贝虫)田鼠DNA。在检测到巴贝虫属的DNA的。和密切相关的泰勒capreoli生物,无论是在H.水蚤蜱发现,新颖奥地利。

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