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首页> 美国卫生研究院文献>3 Biotech >Xylitol production from non-detoxified and non-sterile lignocellulosic hydrolysate using low-cost industrial media components
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Xylitol production from non-detoxified and non-sterile lignocellulosic hydrolysate using low-cost industrial media components

机译：使用低成本工业介质成分从非排毒和非无菌木质纤维素水解物中生产木糖醇

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Immobilized Candida tropicalis cells in freeze dried calcium alginate beads were used for production of xylitol from lignocellulosic waste like corn cob hydrolysate without any detoxification and sterilization of media. Media components for xylitol fermentation were screened by statistical methods. Urea, KH2PO4 and initial pH were identified as significant variables by Plackett–Burman (PB) design. Significant medium components were optimized by response surface methodology (RSM). Predicted xylitol yield by RSM model and experimental yield was 0.87 and 0.79 g/g, respectively. Optimized conditions (urea 1.5 g/L, KH2PO4 1.9 g/L, xylose 55 g/L, pH 6.7) enhanced xylitol yield by 32% and xylose consumption by twofold over those of basal media. In addition, the immobilized cells were reused five times at shake flask level with optimized medium without affecting the xylitol productivity and yield. Xylitol production was successfully scaled up to 7.5 L stirred tank reactor using optimized media. Thus, the optimized condition with non-detoxified pentose hydrolysate from corn cob lignocellulosic waste with minimal nutrients without any sterilization opens up the scope for commercialization of the process.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-017-0700-2) contains supplementary material, which is available to authorized users.
机译：冷冻干燥的藻酸钙珠粒中的固定化热带假丝酵母细胞用于从木质纤维素废料（如玉米芯水解产物）中生产木糖醇，而无需进行任何解毒和培养基灭菌。通过统计方法筛选用于木糖醇发酵的培养基成分。 Plackett-Burman（PB）设计将尿素，KH2PO4和初始pH值确定为重要变量。通过响应面方法（RSM）优化了重要的培养基成分。通过RSM模型预测的木糖醇收率和实验收率分别为0.87和0.79 g / g。优化的条件（尿素1.5 g / L，KH2PO4 1.9 g / L，木糖55 g / L，pH 6.7）使木糖醇收率提高了32％，木糖消耗量比基础培养基提高了两倍。另外，固定化的细胞在摇瓶中用优化的培养基重复使用五次，而不会影响木糖醇的生产率和产量。使用优化的介质成功地将木糖醇生产规模扩大到7.5 L搅拌釜反应器。因此，用玉米芯木质纤维素废料中的非解毒戊糖水解物以最少的养分，不进行任何灭菌的优化条件为该工艺的商业化开辟了空间。电子补充材料本文的在线版本（doi：10.1007 / s13205-017-0700） -2）包含补充材料，授权用户可以使用。

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