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首页> 美国卫生研究院文献>Journal of Bacteriology >Growth Phases of Mycoplasma in Liquid Media Observed with Phase-Contrast Microscope
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Growth Phases of Mycoplasma in Liquid Media Observed with Phase-Contrast Microscope

机译：相差显微镜观察支原体在液体培养基中的生长期

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Razin, Shmuel (University of Connecticut, Storrs), and Benjamin J. Cosenza. Growth phases of Mycoplasma in liquid media observed with phase-contrast microscope. J. Bacteriol. >91:858–869. 1966—Growth of 11 Mycoplasma strains in liquid media was followed by phase-contrast microscopy. A similar pattern of development was common to all strains. Branching filaments, 0.3 to 0.4 μ thick, characterized the early logarithmic phase of growth. The length of the filaments varied according to the strain tested and the growth medium. Addition of oleic acid to the medium induced the formation of very long filaments by M. laidlawii strain B. Upon aging, the filaments were found to break up into chains of coccoid elements. These chains further fragmented to yield shorter chains and single coccoid elements, which characterized the stationary and decline phases of growth. The size of the coccoid elements increased from 0.3 to 0.4 μ, when formed in the filaments, to 0.6 to 0.8 μ after being released from the chains. Further increase in the size of the cells took place at the decline phase of growth, leading to the formation of very large cells reaching a diameter of 10 to 20 μ. However, these large cells had the appearance of empty vesicles and were apparently nonviable as indicated by viable-count experiments.
机译：Razin，Shmuel（康涅狄格大学，斯托斯分校）和Benjamin J. Cosenza。用相差显微镜观察支原体在液体培养基中的生长期。 J.细菌。 > 91： 858–869。 1966年-通过相差显微镜观察了液体培养基中11种支原体菌株的生长。所有菌株都具有相似的发育模式。 0.3至0.4μ厚的分支细丝表征了生长的早期对数阶段。细丝的长度根据所测试的应变和生长培养基而变化。向培养基中添加油酸会引起莱德莫拉氏菌菌株B形成很长的细丝。老化后，发现细丝会分解成球状元素链。这些链进一步断裂，产生较短的链和单个球状元素，这是生长的静止和下降阶段的特征。当在长丝中形成时，球形的尺寸从0.3μm增加到0.4μm，从链中释放出来后增加到0.6μm至0.8μm。细胞大小的进一步增加在生长的下降阶段发生，导致形成直径达到10至20μ的非常大的细胞。然而，这些大细胞具有空泡的外观，并且显然是不可行的，如活菌计数实验所示。

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