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Comparative Electrophoretic and Amino Acid Analyses of Isolated Membranes from Streptococcus pyogenes and Stabilized L-Form

机译:化脓性链球菌和稳定L型分离膜的电泳和氨基酸比较分析

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摘要

Major quantitative, but not qualitative, differences in the various species of proteins in purified membranes from Streptococcus pyogenes and its stabilized L-form have been demonstrated by acidic and alkaline disc gel electrophoresis with and without urea. The fact that no significant differences in the amino acid content or composition between these two membranes could be demonstrated emphasizes that these results are probably due to changes in the relative amounts of the various species of proteins in this subcellular component. The possibility of these protein changes in the L-form membrane being related to its inability to synthesize a rigid cell wall is discussed. Finally, phage-associated lysin, routinely used for removal of the group A streptococcal cell wall, does not appear to affect the protein profile or amino acid composition of the membrane either metabolically or nonmetabolically.
机译:化脓性链球菌纯化膜中各种蛋白质及其稳定的L型在蛋白质上的主要定量差异,但没有定性,已通过在有或没有尿素的情况下进行酸性和碱性圆盘凝胶电泳证实。可以证明这两个膜之间的氨基酸含量或组成没有显着差异的事实强调,这些结果可能是由于该亚细胞成分中各种蛋白质相对含量的变化所致。讨论了L型膜中这些蛋白质变化的可能性与其无法合成刚性细胞壁有关。最后,通常用于去除A组链球菌细胞壁的噬菌体相关溶素似乎在代谢或非代谢方面均不影响膜的蛋白质谱或氨基酸组成。

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