Mass Processing—An Improved Technique for Protein Identification with Mass Spectrometry Data

机译：批量处理—一种利用质谱数据鉴定蛋白质的改进技术

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摘要

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis following tryptic digestion of polyacrylamide gel pieces is a common technique used to identify proteins. This approach is rapid, sensitive, and user friendly, and is becoming widely available to scientists in a variety of biological fields. Here we introduce a simple and effective strategy called “mass processing” where the list of masses generated from a mass spectrometer undergoes two stages of data reduction before identification. Mass processing improves the ability to identify in-gel tryptic-digested proteins by reducing the number of nonsample masses submitted to protein identification database search engines. Our results demonstrate that mass processing improves the statistical score and rank of putative protein identifications, especially with low-quantity samples, thus increasing the ability to confidently identify proteins with mass spectrometry data.

机译：胰蛋白酶消化聚丙烯酰胺凝胶碎片后的基质辅助激光解吸/电离飞行时间质谱分析是用于鉴定蛋白质的常用技术。这种方法是快速，灵敏且用户友好的，并且正被各种生物学领域的科学家广泛使用。在这里，我们介绍一种称为“质量处理”的简单有效的策略，其中质谱仪生成的质量列表在进行识别之前要经历两个阶段的数据缩减。大量处理通过减少提交给蛋白质鉴定数据库搜索引擎的非样品质量的数量，提高了鉴定凝胶中胰蛋白酶消化的蛋白质的能力。我们的结果表明，大规模加工可提高推定蛋白质鉴定的统计评分和等级，尤其是对于低定量样品，从而提高了用质谱数据自信地鉴定蛋白质的能力。

著录项

期刊名称 Journal of Biomolecular Techniques : JBT
作者
Josh A. Henkin; Mark E. Jennings; Dwight E. Matthews; Jim O. Vigoreaux;
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作者单位

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年(卷),期 2004(15),4
年度 2004
页码 230–237
总页数 8
原文格式 PDF
正文语种
中图分类生物学;
关键词
peptide mass fingerprint protein gel electrophoresis MALDI-TOF Drosophila;

机译：肽质量指纹图谱;蛋白质凝胶电泳;MALDI-TOF;果蝇;

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专利

1. Preprocessing Significantly Improves the Peptide/Protein Identification Sensitivity of High-resolution Isobarically Labeled Tandem Mass Spectrometry Data [J] . Sheng Quanhu, Li Rongxia, Dai Jie, Molecular & cellular proteomics: MCP . 2015,第2期

机译：预处理显着提高了高分辨率等压标记串联质谱数据的肽/蛋白质鉴定灵敏度
2. Identification of Metabolites of 6 '-Hydroxy-3,4,5,2 ',4 '-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques [J] . Liu Siyi, Che Yanyun, Wang Fei, Molecules . 2016,第10期

机译：超高效液相色谱-线性离子阱-轨道阱质谱结合多种数据处理技术鉴定大鼠6'-羟基-3,4,5,2'，4'-五甲氧基查尔酮
3. Identification of Metabolites of 6′-Hydroxy-3,4,5,2′,4′-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques [J] . Siyi Liu, Yanyun Che, Fei Wang, Molecules . 2016,第10期

机译：基于多种数据处理技术的超高效液相色谱-线性离子阱-轨道阱质谱联用鉴定大鼠6'-羟基-3,4,5,2'，4'-五甲氧基查尔酮
4. Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectromet [C] . Daisy-Malloy Hamburg, Moo-Jin Suh, Patrick A. Limbach International Symposium on Runaway Reactions, Pressure Relief Design and Effluent Handling American Institute of Chemical Engineers/American Chemical Society Management Conference . 2005

机译：通过基质辅助激光解吸/电离质谱法通过基质辅助激光解吸/电离质谱鉴定通过基质辅助激光解吸/电离质谱鉴定蛋白质中的蛋白质中蛋白质的鉴定
5. Separation and identification techniques for membrane proteins using ultra-high pressure liquid chromatography coupled to mass spectrometry. [D] . Moore, Stephanie Marie. 2016

机译：使用超高压液相色谱与质谱联用的膜蛋白分离和鉴定技术。
6. Identification of Metabolites of 6′-Hydroxy-3452′4′-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques [O] . Siyi Liu, Yanyun Che, Fei Wang, 2016

机译：基于多种数据处理技术的超高效液相色谱-线性离子阱-轨道阱质谱联用鉴定大鼠6-羟基-34524-五甲氧基查尔酮
7. Identification of Metabolites of 6′-Hydroxy-3,4,5,2′,4′-pentamethoxychalcone in Rats by a Combination of Ultra-High-Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry Based on Multiple Data Processing Techniques [O] . Siyi Liu, Yanyun Che, Fei Wang, 2016

机译：基于多种数据处理技术的超高效液相色谱 - 线性离子阱 - 轨道阱质谱联用鉴定大鼠6'-羟基-3,4,5,2'，4'-五甲氧基查尔酮的代谢产物
8. Identification of Two Glucuronide Metabolites of Doxylamine via Thermospray/Mass Spectrometry and Thermospray/Mass Spectrometry/Mass Spectrometry [R] . Korfmacher, W. A. , Holder, C. L. , Betowski, L. D. , 1987

机译：通过热阵/质谱和热/质谱/质谱鉴定多西拉敏的两个葡萄糖醛酸代谢物

Mass Processing—An Improved Technique for Protein Identification with Mass Spectrometry Data

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