首页> 美国卫生研究院文献>Journal of Bacteriology >Specialized lambda transducing bacteriophage which carries hisS the structural gene for histidyl-transfer ribonucleic acid synthetase.
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Specialized lambda transducing bacteriophage which carries hisS the structural gene for histidyl-transfer ribonucleic acid synthetase.

机译:带有hisS的专门的λ转导噬菌体hisS是组氨酸转移的核糖核酸合成酶的结构基因。

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摘要

A number of specialized lambda transducing bacteriophages which carry the Escherichia coli gene guaB were isolated from E. coli. One of these bacteriophages, lambda cI857 Sam7 d guaB-2, also carries hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase (EC 6.1.1.21). Histidyl-transfer ribonucleic acid synthetase activities in induced and uninduced lysogens carrying lambda d guaB-2 indicate that the phage carries the entire structural gene and that the gene is under the control of an E. coli promoter. These conclusions were confirmed by the in vivo production of a protein encoded by the phage which comigrates with authentic histidyl-transfer ribonucleic acid synthetase on two-dimensional polyacrylamide gels.
机译:从大肠杆菌中分离出许多携带大肠杆菌基因guaB的专门的λ转导噬菌体。这些噬菌体中的一种,λcI857 Sam7 d guaB-2,也带有hisS,组氨酸转移核糖核酸合成酶的结构基因(EC 6.1.1.21)。在携带lamb d guaB-2的诱导和未诱导溶原菌中的组氨酸转移核糖核酸合成酶活性表明,噬菌体携带整个结构基因,并且该基因处于大肠杆菌启动子的控制之下。这些结论被在噬菌体编码的蛋白质的体内产生所证实,该蛋白质与二维聚丙烯酰胺凝胶上的真实的组氨酸转移核糖核酸合成酶竞争。

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