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首页> 美国卫生研究院文献>Journal of Bacteriology >Cysteine biosynthesis in Saccharomyces cerevisiae: mutation that confers cystathionine beta-synthase deficiency.
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Cysteine biosynthesis in Saccharomyces cerevisiae: mutation that confers cystathionine beta-synthase deficiency.

机译：酿酒酵母中的半胱氨酸生物合成：赋予胱硫醚β-合酶缺陷的突变。

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摘要

The cys2-1 mutation of Saccharomyces cerevisiae was originally thought to confer cysteine dependence through a serine O-acetyltransferase deficiency. In this study, we show that cys2-1 strains lack not only serine O-acetyltransferase but also cystathionine beta-synthase. However, a prototrophic strain was found to be serine O-acetyltransferase deficient because of a mutation allelic to cys2-1. Moreover, revertants obtained from cys2-1 strains had serine O-acetyltransferase but not cystathionine beta-synthase, whereas transformants obtained by treating a cys2-1 strain with an S. cerevisiae genomic library had cystathionine beta-synthase but not serine O-acetyltransferase. From these observations, we conclude that cys2-1 (serine O-acetyltransferase deficiency) accompanies a very closely linked mutation that causes cystathionine beta-synthase deficiency and that these mutations together confer cysteine dependence. This newly identified mutation is named cys4-1. These results not only support our previous hypothesis that S. cerevisiae has two functional cysteine biosynthetic pathways but also reveal an interesting gene arrangement of the cysteine biosynthetic system.
机译：最初认为酿酒酵母的cys2-1突变通过丝氨酸O-乙酰基转移酶缺陷赋予半胱氨酸依赖性。在这项研究中，我们显示cys2-1菌株不仅缺少丝氨酸O-乙酰基转移酶，而且还缺少胱硫醚β-合酶。但是，发现原养型菌株由于cys2-1等位基因突变而缺乏丝氨酸O-乙酰基转移酶。此外，从cys2-1菌株获得的回复株具有丝氨酸O-乙酰基转移酶，但没有胱硫醚β-合酶，而通过用酿酒酵母基因组文库处理cys2-1菌株而获得的转化体具有胱硫醚β-合酶，但没有丝氨酸O-乙酰基转移酶。从这些观察结果，我们得出结论，cys2-1（丝氨酸O-乙酰基转移酶缺陷）伴随着一个非常紧密相关的突变，导致胱硫醚β-合酶缺陷，并且这些突变共同赋予了半胱氨酸依赖性。这个新发现的突变称为cys4-1。这些结果不仅支持我们先前的假设，即酿酒酵母具有两个功能性半胱氨酸生物合成途径，而且还揭示了半胱氨酸生物合成系统的有趣基因排列。

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