• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Bacteriology >Site-specific integration in Saccharopolyspora erythraea and multisite integration in Streptomyces lividans of actinomycete plasmid pSE101.
【2h】

Site-specific integration in Saccharopolyspora erythraea and multisite integration in Streptomyces lividans of actinomycete plasmid pSE101.

机译：在Saccharopolyspora erythraea中进行位点特异性整合在放线菌质粒pSE101的lividans链霉菌中进行多位点整合。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An 11.3-kilobase-pair plasmid, designated pSE101, exists in Saccharopolyspora erythraea NRRL 2338 as an integrated sequence (pSE101int) at a unique chromosomal location and in the free form in less than an average of 1 copy per 10 chromosomes. The plasmid sequence is missing from S. erythraea NRRL 2359. Restriction maps of the free and integrated forms of pSE101 showed point-to-point correspondence. Plasmid pECT2 was constructed by ligation of pSE101, pBR322, and the gene for thiostrepton resistance (tsr). When introduced by polyethylene glycol-mediated transformation into protoplasts of S. erythraea NRRL 2359, all thiostrepton-resistant regenerants examined were found to carry a single copy of pECT2 in the integrated state at a single chromosomal site. The chromosomal site of pECT2 integration in strain NRRL 2359 (attB) corresponded to the chromosomal location of pSE101int in strain NRRL 2338. The plasmid crossover site (attP) was mapped to the plasmid site that corresponded to the site of interruption of the plasmid sequence in the host carrying pSE101int, indicating that site-specific integrative recombination had occurred. An additional 2.8-kilobase-pair chromosomal sequence homologous to a segment of pSE101 was also observed in strains NRRL 2338 and NRRL 2359. After introduction of pECT2 into Streptomyces lividans, approximately half of the transformants examined were found to carry the plasmid as a stable, autonomously replicating element. The other half carried a single copy of pECT2 as an integrated sequence, but the location of pECT2int in Streptomyces lividans varied from one transformant to another. In each case, integrative crossover used the attP site. A model is proposed to account for the determination of the particular state of pSE101 in Streptomyces lividans.
机译：一种名为pSE101的11.3碱基对质粒，在整合的序列（pSE101int）中位于独特的染色体位置，以游离序列形式存在于红糖酵母NRRL 2338中，每10条染色体平均少于1个拷贝。红细菌链霉菌NRRL 2359中缺少质粒序列。pSE101游离形式和整合形式的限制性酶切图显示了点对点对应。通过pSE101，pBR322和硫代链霉菌抗性基因（tsr）的连接构建质粒pECT2。当通过聚乙二醇介导的转化将其引入到S. erythraea NRRL 2359的原生质体中时，发现所检查的所有抗硫链丝菌抗性再生剂均在单个染色体位点上以整合状态携带单个拷贝的pECT2。 NRRL 2359（attB）菌株中pECT2整合的染色体位点与NRRL 2338菌株pSE101int的染色体位置相对应。质粒交换位点（attP）被映射到与质粒序列中断的位点相对应的质粒位点。携带pSE101int的宿主，表明发生了位点特异性整合重组。在NRRL 2338和NRRL 2359菌株中还观察到了与pSE101片段同源的另一个2.8碱基对染色体序列。在将pECT2导入链霉菌链霉菌后，发现大约一半的转化体可以稳定地携带该质粒，自主复制元素。另一半携带pECT2的单个拷贝作为整合序列，但是pECT2int在淡链霉菌中的位置从一个转化体到另一个转化体而变化。在每种情况下，集成交叉都使用了attP站点。提出了一个模型来解释确定链霉菌中pSE101的特定状态。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号