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首页> 美国卫生研究院文献>3 Biotech >Isolation characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)
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Isolation characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)

机译：辣椒多病毒抗性系NBS-LRR类抗病基因类似物的分离鉴定和遗传多样性

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摘要

Viruses are serious threat to chilli crop production worldwide. Resistance screening against several viruses resulted in identifying a multiple virus resistant genotype ‘IHR 2451’. Degenerate primers based on the conserved regions between P-Loop and GLPL of Resistance genes (R-genes) were used to amplify nucleotide binding sites (NBS)—encoding regions from genotype ‘IHR 2451’. Alignment of deduced amino acid sequences and phylogenetic analyses of isolated sequences distinguished into two groups representing toll interleukin-1 receptor (TIR) and non-TIR, and different families within the group confirming the hypotheses that dicots have both the types of NBS-LRR genes. The alignment of deduced amino acid sequences revealed conservation of subdomains P-loop, RNBS-A, kinase2, RNBS-B, and GLPL. The distinctive five RGAs showing specific conserved motifs were subjected to BLASTp and indicated high homology at deduced amino acid level with R genes identified such as Pvr9 gene for potyvirus resistance, putative late blight resistance protein homolog R1B-23 and other disease resistance genes suggesting high correlation with resistance to different pathogens. These pepper RGAs could be regarded as candidate sequences of resistant genes for marker development.
机译：病毒对全世界的辣椒作物生产构成严重威胁。对几种病毒的抗性筛选导致鉴定出多种抗病毒基因型“ IHR 2451”。基于抗性基因（R基因）的P-Loop和GLPL之间的保守区域的简并引物被用于扩增核苷酸结合位点（NBS），即基因型“ IHR 2451”的编码区域。推导的氨基酸序列的比对和分离的序列的系统发育分析，分为收费白介素-1受体（TIR）和非TIR两个组，该组中的不同家族证实了双子叶植物具有NBS-LRR基因的两种类型的假设。推导的氨基酸序列的比对揭示了亚结构域P-环，RNBS-A，激酶2，RNBS-B和GLPL的保守性。对五个显示特定保守基序的独特RGA进行BLASTp处理，并在推导的氨基酸水平上与R基因鉴定出高度同源性，这些R基因已鉴定为对马铃薯病毒具有抗性的Pvr9基因，假定的晚疫病抗性蛋白质同源物R1B-23和其他表明高度相关的抗病基因对不同病原体有抵抗力。这些胡椒RGA可以被认为是用于标记开发的抗性基因的候选序列。

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