Single-Molecule Imaging and Spectroscopy Using Fluorescence and Surface-Enhanced Raman Scattering

机译：使用荧光和表面增强拉曼散射的单分子成像和光谱

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摘要

We extended single molecule fluorescence imaging and time-resolved fluorometry from the green to the violet-excitation regime to find feasibility of detecting and identifying fluorescent analogs of nucleic-acid bases at the single-molecule level. Using violet excitation, we observed fluorescent spotsfrom single complexes composed of a nucleotide analogue and the Klenow fragmentof DNA polymerase I. Also, we implemented Raman imaging and spectroscopy of adenine molecules adsorbed on Ag colloidal nanoparticles to find feasibility of identifying nucleic-acid bases at the single-molecule level. Surface enhanced Raman scattering (SERS) of adenine molecules showed an intermittent on-and-off behavior called blinking. The observation of blinking provides substantial evidence for detecting single adenine molecules.

机译：我们将单分子荧光成像和时间分辨荧光法从绿色激发到紫色激发机制，以发现检测和鉴定单分子水平核酸碱基的荧光类似物的可行性。使用紫光激发，我们观察到了由核苷酸类似物和DNA聚合酶I的Klenow片段组成的单个复合物的荧光斑点。此外，我们对吸附在Ag胶体纳米颗粒上的腺嘌呤分子进行了拉曼成像和光谱学分析，以发现在核酸胶体上鉴定核酸碱基的可行性。单分子水平。腺嘌呤分子的表面增强拉曼散射（SERS）显示出一种称为眨眼的间歇性开/关行为。眨眼的观察为检测单个腺嘌呤分子提供了实质性证据。

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期刊名称 Journal of Biological Physics
作者
M. Ishikawa; Y. Maruyama; J.-Y. Ye; M. Futamata;
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作者单位

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年(卷),期 2002(28),4
年度 2002
页码 573–585
总页数 14
原文格式 PDF
正文语种
中图分类生物学;
关键词
adenine fluorescence Klenow fragment silver particles single molecule imaging surface-enhanced Raman scattering TNP-ATP;

机译：腺嘌呤;荧光;Klenow片段;银颗粒;单分子成像;表面增强拉曼散射;TNP-ATP;

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专利

1. Single-Molecule Imaging and Spectroscopy Using Fluorescence and Surface-Enhanced Raman Scattering [J] . M. Ishikawa, Y. Maruyama, J.-Y. Ye, Journal of Biological Physics . 2002,第4期

机译：使用荧光和表面增强拉曼散射的单分子成像和光谱
2. Surface-enhanced Raman scattering in local optical fields of silver and gold nanoaggregatess - From single-molecule Raman spectroscopy to ultrasensitive probing in live cells [J] . Kneipp K, Kneipp H, Kneipp J Accounts of Chemical Research . 2006,第7期

机译：银和金纳米聚集体局部光场中的表面增强拉曼散射-从单分子拉曼光谱到活细胞的超灵敏探测
3. Single-Molecule Surface-Enhanced Raman and Fluorescence Correlation Spectroscopy of Horseradish Peroxidase [J] . Erik J. Bjerneld, Zeno Foldes-Papp, Mikael Kall, The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2002,第6期

机译：辣根过氧化物酶的单分子表面增强拉曼光谱和荧光相关光谱
4. Engineering nanostructures for single-molecule surface-enhanced Raman spectroscopy [C] . M. Moskovits, Dae-Hong Jeong, Tsachi Livneh, Nanosensing: Materials and Devices . 2004

机译：用于单分子表面增强拉曼光谱的工程纳米结构
5. Surface-enhanced Raman scattering and surface-enhanced hyper Raman scattering: A systematic study of various probing molecules on novel substrates. [D] . Huang, Qunjian. 2003

机译：表面增强的拉曼散射和表面增强的超拉曼散射：对新型基质上各种探测分子的系统研究。
6. Molecular imaging of atherosclerosis: spotlight on Raman spectroscopy and surface-enhanced Raman scattering [O] . Neil MacRitchie, Gianluca Grassia, Jonathan Noonan, -1

机译：动脉粥样硬化的分子成像：拉曼光谱和表面增强拉曼散射的聚光灯
7. Developing the Techniques for Raman, Surface-Enhanced Raman Scattering (SERS), and Fluorescence Spectroscopies of Organic Molecules in Isolation [O] . Wolfe Jonathan 2010

机译：开发用于分离中的有机分子的拉曼，表面增强拉曼散射（SERS）和荧光光谱的技术
8. Surface-Enhanced Raman Spectroscopy of Electrochemically Characterized Interfaces. Relations between Raman Scattering Intensity and Surface Coverage for Simple Anionic Adsorbates [R] . Weaver, M. J., Hupp, J. T., Barz, F., 1983

机译：电化学表征界面的表面增强拉曼光谱。简单阴离子吸附物的拉曼散射强度与表面覆盖率的关系

Single-Molecule Imaging and Spectroscopy Using Fluorescence and Surface-Enhanced Raman Scattering

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