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首页> 美国卫生研究院文献>Journal of Bacteriology >Involvement of methyltransferase-activating protein and methyltransferase 2 isoenzyme II in methylamine:coenzyme M methyltransferase reactions in Methanosarcina barkeri Fusaro.
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Involvement of methyltransferase-activating protein and methyltransferase 2 isoenzyme II in methylamine:coenzyme M methyltransferase reactions in Methanosarcina barkeri Fusaro.

机译：甲基转移酶激活蛋白和甲基转移酶2同工酶II参与甲烷化甲烷菌（Methanosarcina barkeri Fusaro）的甲胺：辅酶M甲基转移酶反应。

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摘要

The enzyme systems involved in the methyl group transfer from methanol and from tri- and dimethylamine to 2-mercaptoethanesulfonic acid (coenzyme M) were resolved from cell extracts of Methanosarcina barkeri Fusaro grown on methanol and trimethylamine, respectively. Resolution was accomplished by ammonium sulfate fractionation, anion-exchange chromatography, and fast protein liquid chromatography. The methyl group transfer reactions from tri- and dimethylamine, as well as the monomethylamine:coenzyme M methyltransferase reaction, were strictly dependent on catalytic amounts of ATP and on a protein present in the 65% ammonium sulfate supernatant. The latter could be replaced by methyltransferase-activating protein isolated from methanol-grown cells of the organism. In addition, the tri- and dimethylamine:coenzyme M methyltransferase reactions required the presence of a methylcobalamin:coenzyme M methyltransferase (MT2), which is different from the analogous enzyme from methanol-grown M. barkeri. In this work, it is shown that the various methylamine:coenzyme M methyltransfer steps proceed in a fashion which is mechanistically similar to the methanol:coenzyme M methyl transfer, yet with the participation of specific corrinoid enzymes and a specific MT2 isoenzyme.
机译：从分别在甲醇和三甲胺上生长的巴氏甲烷八叠球菌的细胞提取物中分离出涉及甲基从甲醇以及从三胺和二甲胺到2-巯基乙磺酸的甲基转移酶系统（辅酶M）。通过硫酸铵分级分离，阴离子交换色谱和快速蛋白质液相色谱完成分离。来自三胺和二甲基胺的甲基转移反应以及单甲胺：辅酶M甲基转移酶反应严格取决于ATP的催化量以及65％硫酸铵上清液中存在的蛋白质。后者可以用从生物体的甲醇生长细胞中分离的甲基转移酶激活蛋白代替。另外，三和二甲胺：辅酶M甲基转移酶反应需要存在甲基钴胺素：辅酶M甲基转移酶（MT2），该酶不同于甲醇生长的巴氏支原体。在这项工作中，显示了各种甲胺：辅酶M甲基转移步骤以与甲醇：辅酶M甲基转移机理类似的方式进行，但是有特定的类rinrinoid酶和特定的MT2同工酶参与。

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