首页> 美国卫生研究院文献>Journal of Bacteriology >Note: Cloning and Sequencing of a Form II Ribulose-15-Bisphosphate Carboxylase/Oxygenase from the Bacterial Symbiont of the Hydrothermal Vent Tubeworm Riftia pachyptila
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Note: Cloning and Sequencing of a Form II Ribulose-15-Bisphosphate Carboxylase/Oxygenase from the Bacterial Symbiont of the Hydrothermal Vent Tubeworm Riftia pachyptila

机译:注意:克隆和测序的形式从热液性口气管虫Riftia pachyptila细菌共生体的15-双磷酸核糖-15-二磷酸羧化酶/加氧酶。

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摘要

The bacterial symbiont of the hydrothermal vent tubeworm fixes carbon via the Calvin-Benson cycle and has been shown previously to express a form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene cbbM, which encodes this enzyme, has been cloned and sequenced. The gene has the highest identity with the cbbM gene from Rhodospirillum rubrum, and analysis of the inferred amino acid sequence reveals that all active-site residues are conserved. This is the first form II RubisCO cloned and sequenced from a chemoautotrophic symbiont and from a deep-sea organism.
机译:热液喷发tube虫的细菌共生体通过卡尔文-本森循环固定碳,先前已显示其表达II型核糖-1,5-双磷酸羧化酶/加氧酶(RubisCO)。已经克隆并测序了编码该酶的基因cbbM。该基因与红红螺螺旋体的cbbM基因具有最高的同一性,对推测的氨基酸序列的分析表明,所有活性位点残基均被保守。这是从化学自养共生体和深海生物中克隆并测序的第一种形式II RubisCO。

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