• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Bacteriology >Cloning and Expression of the algL Gene Encoding the Azotobacter chroococcum Alginate Lyase: Purification and Characterization of the Enzyme
【2h】

Cloning and Expression of the algL Gene Encoding the Azotobacter chroococcum Alginate Lyase: Purification and Characterization of the Enzyme

机译：编码绿色固氮藻藻酸裂解酶的algL基因的克隆和表达：酶的纯化和表征

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a potential N-terminal signal peptide that is consistent with its proposed periplasmic location. The analysis of the deduced amino acid sequence indicated that the gene sequence has a high homology (90% identity) to the Azotobacter vinelandii gene sequence, which has very recently been deposited in the GenBank database, and that it has 64% identity to the Pseudomonas aeruginosa gene sequence but that it has rather low homology (15 to 22% identity) to the gene sequences encoding alginate lyase in other bacteria. The A. chroococcum AlgL protein was overproduced in Escherichia coli and purified to electrophoretic homogeneity in a two-step chromatography procedure on hydroxyapatite and phenyl-Sepharose. The kinetic and molecular parameters of the recombinant alginate lyase are similar to those found for the native enzyme.
机译：绿脓杆菌的藻酸盐裂解酶编码基因（algL）定位于一个3.1kb EcoRI DNA片段，该片段揭示了一个1,116 bp的开放阅读框。该开放阅读框编码42.98 kDa的蛋白质，与我们先前报道的该蛋白质的值一致。推导的蛋白质具有潜在的N末端信号肽，与其建议的周质定位相符。对推导的氨基酸序列的分析表明，该基因序列与Azotobacter vinelandii基因序列具有很高的同源性（90％同源性），该序列最近已保存在GenBank数据库中，并且与假单胞菌具有64％的同源性。铜绿假单胞菌基因序列，但与其他细菌中编码藻酸盐裂解酶的基因序列具有相当低的同源性（同一性为15％至22％）。在大肠杆菌中过量生产嗜铬曲霉AlgL蛋白，并在两步色谱法中在羟基磷灰石和苯基-琼脂糖上纯化至电泳均一。重组藻酸盐裂解酶的动力学和分子参数与天然酶相似。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号