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Mapping the MinE Site Involved in Interaction with the MinD Division Site Selection Protein of Escherichia coli

机译:映射与大肠杆菌的MinD分区位点选择蛋白相互作用的MinE位点

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摘要

Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum. The site within MinE that is required for interaction with MinD was mapped by studying the effects of site-directed minE mutations on MinD-MinE interactions in yeast two-hybrid and three-hybrid experiments. This confirmed that the MinE N-terminal domain is responsible for the interaction of MinE with MinD. Mutations that interfered with the interaction defined an extended surface on one face of the α-helical region of the MinE N-terminal domain, consistent with the idea that the MinE-MinD interaction involves formation of a coiled-coil structure by interaction with a complementary helical surface within MinD.
机译:MinD和MinE蛋白之间的相互作用是正确放置大肠杆菌分裂间隔所需的。通过研究酵母两杂交和三杂交实验中定点的minE突变对MinD-MinE相互作用的影响,可以绘制与MinD相互作用所需的MinE中的位点。这证实了MinE N末端域负责MinE与MinD的相互作用。干扰相互作用的突变在MinE N末端结构域的α螺旋区域的一个面上定义了一个扩展的表面,这与MinE-MinD相互作用涉及通过与互补序列相互作用形成卷曲螺旋结构的想法一致MinD内的螺旋表面。

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