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首页> 美国卫生研究院文献>Journal of Bacteriology >Putative Quorum-Sensing Regulator BlxR of Brucella melitensis Regulates Virulence Factors Including the Type IV Secretion System and Flagella
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Putative Quorum-Sensing Regulator BlxR of Brucella melitensis Regulates Virulence Factors Including the Type IV Secretion System and Flagella

机译：假定布鲁姆菌布鲁姆菌的群体感应调节器BlxR调节包括IV型分泌系统和鞭毛在内的致病因子。

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Brucella melitensis is an intracellular pathogen that establishes a replicative niche within macrophages. While the intracellular lifestyle of Brucella is poorly understood and few virulence factors have been identified, components of a quorum-sensing pathway in Brucella have recently been identified. The LuxR-type regulatory protein, VjbR, and an N-acylhomoserine lactone signaling molecule are both involved in regulating expression of the virB-encoded type IV secretion system. We have identified a second LuxR-type regulatory protein (BlxR) in Brucella. Microarray analysis of a blxR mutant suggests that BlxR regulates the expression of a number of genes, including those encoding the type IV secretion system and flagella. Confirming these results, deletion of blxR in B. melitensis reduced the transcriptional activities of promoters for the virB operon, flagellar genes, and another putative virulence factor gene, bopA. Furthermore, our data suggested that both BlxR and VjbR are positively autoregulated and cross-regulate the expression of each other. The blxR deletion strain exhibited reduced growth in macrophages, similar to that observed for a vjbR deletion strain. However, unlike the vjbR deletion, the blxR deletion did not fully attenuate virulence in mice. More strikingly, bioluminescent imaging revealed that dissemination of the blxR mutant was similar to that of wild-type B. melitensis, while the vjbR mutant was defective for systemic spread in IRF-1−/− mice, suggesting that these regulators are not functionally redundant but that they converge in a common pathway regulating bacterial processes.
机译：布鲁氏菌是一种细胞内病原体，可在巨噬细胞内建立复制性生态位。尽管对布鲁氏菌的细胞内生活方式知之甚少，并且几乎没有发现毒力因子，但最近已经鉴定出布鲁氏菌中群体感应途径的成分。 LuxR型调节蛋白VjbR和N-酰基高丝氨酸内酯信号分子均参与调节virB编码的IV型分泌系统的表达。我们在布鲁氏菌中鉴定出第二个LuxR型调节蛋白（BlxR）。 blxR突变体的微阵列分析表明，BlxR调节许多基因的表达，包括那些编码IV型分泌系统和鞭毛的基因。证实了这些结果，melitensis中blxR的缺失降低了virB操纵子，鞭毛基因和另一个推定的毒力因子基因bopA的启动子的转录活性。此外，我们的数据表明，BlxR和VjbR均被正向自动调节并相互调节彼此的表达。 blxR缺失菌株在巨噬细胞中的生长减少，类似于vjbR缺失菌株。但是，与vjbR缺失不同，blxR缺失并未完全减弱小鼠的毒力。更加惊人的是，生物发光成像显示blxR突变体的传播与野生型 B相似。 melitensis ，而 vjbR 突变体在IRF-1 -/-小鼠中的系统传播存在缺陷，这表明这些调节子在功能上不是多余的，但它们会收敛在调节细菌过程的常见途径中。

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