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首页> 美国卫生研究院文献>Marine Drugs >LC/MS Analysis of Tetrodotoxin and Its Deoxy Analogs in the Marine Puffer Fish Fugu niphobles from the Southern Coast of Korea and in the Brackishwater Puffer Fishes Tetraodon nigroviridis and Tetraodon biocellatus from Southeast Asia
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LC/MS Analysis of Tetrodotoxin and Its Deoxy Analogs in the Marine Puffer Fish Fugu niphobles from the Southern Coast of Korea and in the Brackishwater Puffer Fishes Tetraodon nigroviridis and Tetraodon biocellatus from Southeast Asia

机译：LC / MS分析来自韩国南海岸的河豚河豚鱼和来自东南亚的咸淡水河豚河豚鱼中的河豚毒素和其脱氧类似物

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摘要

Tetrodotoxin (TTX) and its deoxy analogs, 5-deoxyTTX, 11-deoxyTTX, 6,11-dideoxyTTX, and 5,6,11-trideoxyTTX, were quantified in the tissues of three female and three male specimens of the marine puffer fish, Fugu niphobles, from the southern coast of Korea, and in the whole body of the brackishwater puffer fishes, Tetraodon nigroviridis (12 specimens) and Tetrodon biocellatus (three specimens) from Southeast Asia using LC/MS in single ion mode (SIM). Identification of these four deoxy analogs in the ovarian tissue of F. niphobles were further confirmed by LC/MS/MS. TTX and 5,6,11-trideoxyTTX were detected in all three puffer fish species as the major TTX analogs, similar to Japanese Fugu pardalis. While 6,11-dideoxyTTX was also found to be a major analog in almost all tissues of Korean F. niphobles, this analog was minor in the two Tetraodon species and Japanese F. pardalis. Among the tissues of F. niphobles, the concentrations of TTXs were highest in the ovaries (female) and skin (female and male).
机译：在海洋河豚的三只雌性和三只雄性标本的组织中定量了河豚毒素（TTX）及其脱氧类似物5-deoxyTTX，11-deoxyTTX，6,11-dideoxyTTX和5,6,11-trideoxyTTX，来自韩国南部海岸的咸河豚鱼，以及咸淡水河豚鱼的整个体内，来自东南亚的Tetraodon nigroviridis（12个标本）和Tetrodon biocellatus（三个标本）使用LC / MS以单离子模式（SIM）采集。 LC / MS / MS进一步证实了嗜盐镰刀菌卵巢组织中这四种脱氧类似物的鉴定。在所有三种河豚鱼中都检测到了TTX和5,6,11-trideoxyTTX，这是主要的TTX类似物，类似于日本河豚。虽然还发现在朝鲜ni蝶的几乎所有组织中，6,11-dideoxyTTX都是主要的类似物，但在两个四角形物种和日本F中却是次要的。在F. niphobles组织中，TTXs的浓度在卵巢（女性）和皮肤（女性和男性）中最高。

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