首页> 美国卫生研究院文献>Journal of Assisted Reproduction and Genetics >Fertilization and Development of a Blastocyst-Stage Embryo After Selective Intracytoplasmic Sperm Injection of a Mature Oocyte from a Binovular Zona Pellucida: A Case Report
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Fertilization and Development of a Blastocyst-Stage Embryo After Selective Intracytoplasmic Sperm Injection of a Mature Oocyte from a Binovular Zona Pellucida: A Case Report

机译:选择性胞浆内精子注射双卵泡透明带成熟卵母细胞的胚泡期胚的受精和发育:一例报告。

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摘要

>Purpose:Our purpose is to describe the development of a blastocyst-stage embryo after the selective fertilization of a mature oocyte from a binovular zona pellucida by intracytoplasmic sperm injection (ICSI).>Method:A 34-year-old woman underwent intracytoplasmic sperm injection due to severe male-factor infertility. After oocyte retrieval, a binovular zona pellucida including one mature metaphase II oocyte and one immature oocyte at the germinal vesicle stage as well as nine metaphase II oocytes was injected with spermatozoa using a one-to-one approach.>Results:The injected mature oocyte of the binovular zona pellucida showed fertilization as evidenced by the presence of two pronuclei and cleaved to a four-cell embryo on the second day, while the uninjected oocyte showed signs of degeneration. On the third day, this embryo further cleaved to six blastomeres with slight fragmentation and it reached the blastocyst stage on the sixth day.>Conclusions:Selective fertilization of one oocyte from a binovular zona pellucida by ICSI may lead to the development of a morphologically normal blastocyst-stage embryo which can be used for embryo transfer in the presence of a limited number of embryos.
机译:>目的:我们的目的是描述通过胞浆内精子注射(ICSI)对双卵透明带的成熟卵母细胞进行选择性受精后胚泡期胚胎的发育。>方法:由于严重的男性不育症,一名34岁妇女接受了胞浆内精子注射。取卵后,采用一对一的方法向双生卵透明带中注入精子,其中包括一个成熟的中期II卵母细胞和一个在生小泡期的不成熟卵母细胞,以及9个中期II卵母细胞。>结果:注入的双卵透明带成熟卵母细胞显示受精,第二天存在两个原核并分裂成四个细胞的胚胎,而未注入卵母细胞则显示出变性迹象。在第三天,该胚胎进一步分裂成六个卵裂球,并有轻微碎片,并在第六天到达胚泡阶段。>结论: ICSI对双卵透明带中的一个卵母细胞进行选择性受精可能导致形态正常的胚泡期胚胎的发育,可在有限数量的胚胎存在下用于胚胎移植。

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