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首页> 美国卫生研究院文献>Marine Drugs >Transcriptional Changes Caused by Bisphenol A in Oryzias javanicus a Fish Species Highly Adaptable to Environmental Salinity
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Transcriptional Changes Caused by Bisphenol A in Oryzias javanicus a Fish Species Highly Adaptable to Environmental Salinity

机译：双酚A引起的爪哇稻（Oryzias javanicus）（一种高度适应环境盐度的鱼类）的转录变化

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摘要

The Javanese medaka, Oryzias javanicus, is a fish highly adaptable to various environmental salinities. Here, we investigated the effects of the environmental pollutant bisphenol A (BPA; an endocrine disrupting chemical) on gene expression levels in this species acclimated to different salinities. Using cDNA microarrays, we detected the induction of differential expression of genes by BPA, and compared the transcriptional changes caused by chemical exposure at different salinities. There were marked transcriptional changes induced by BPA between treatments. While 533 genes were induced by a factor of more than two when O. javanicus was exposed to BPA in seawater, only 215 genes were induced in freshwater. Among those genes, only 78 were shared and changed significantly their expression in both seawater and freshwater. Those genes were mainly involved in cellular processes and signaling pathway. We then categorized by functional group genes specifically induced by BPA exposure in seawater or freshwater. Gene expression changes were further confirmed in O. javanicus exposed to various concentrations of BPA, using quantitative real-time reverse transcription PCR based on primer sets for 28 selected genes.
机译：爪哇（Oryzias javanicus）是一种高度适应各种环境盐度的鱼类。在这里，我们研究了环境污染物双酚A（BPA；一种内分泌干扰化学物质）对适应不同盐度的该物种基因表达水平的影响。使用cDNA微阵列，我们检测到BPA诱导基因差异表达，并比较了在不同盐度下化学暴露引起的转录变化。在两次治疗之间，BPA诱导了明显的转录变化。当爪哇O. javanicus暴露于海水中的BPA时，有533个基因被诱导超过两倍，而在淡水中仅诱导了215个基因。在这些基因中，只有78个被共享，并且在海水和淡水中均显着改变了它们的表达。这些基因主要参与细胞过程和信号通路。然后，我们根据由BPA暴露在海水或淡水中特异性诱导的功能基团基因进行分类。使用基于28个选定基因的引物集的定量实时逆转录PCR，在暴露于各种浓度BPA的爪哇稻中进一步证实了基因表达的变化。

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