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首页> 美国卫生研究院文献>3 Biotech >Evaluating water deficit and glyphosate treatment on the accumulation of phenolic compounds and photosynthesis rate in transgenic Codonopsis lanceolata (Siebold Zucc.) Trautv. over-expressing γ-tocopherol methyltransferase (γ-tmt) gene
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Evaluating water deficit and glyphosate treatment on the accumulation of phenolic compounds and photosynthesis rate in transgenic Codonopsis lanceolata (Siebold Zucc.) Trautv. over-expressing γ-tocopherol methyltransferase (γ-tmt) gene

机译：评价水分亏缺和草甘膦处理对轮叶党参党参中酚类化合物积累和光合作用速率的影响。过度表达γ-生育酚甲基转移酶（γ-tmt）基因

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The effect of water stress and herbicide treatment on the phenolic compound concentration and photosynthesis rate in transgenic Codonopsis lanceolata plants over-expressing the γ-tmt gene was investigated and compared to that in control non-transgenic C. lanceolata plants. The total phenolic compound content was investigated using high-performance liquid chromatography combined with diode array detection in C. lanceolata seedlings 3 weeks after water stress and treatment with glyphosate. Changes in the composition of phenolic compounds were observed in leaf and root extracts from transformed C. lanceolata plants following water stress and treatment with glyphosate. The total concentration of phenolic compounds in the leaf extracts of transgenic samples after water stress ranged from 3455.13 ± 40.48 to 8695.00 ± 45.44 µg g−1 dry weight (DW), whereas the total concentration phenolic compound in the leaf extracts of non-transgenic control samples was 5630.83 ± 45.91 µg g−1 DW. The predominant phenolic compounds that increased after the water stress in the transgenic leaf were (+) catechin, benzoic acid, chlorogenic acid, ferulic acid, gallic acid, rutin, vanillic acid, and veratric acid. The total concentration of phenolic compounds in the leaf extracts of transgenic samples after glyphosate treatment ranged from 4744.37 ± 81.81 to 12,051.02 ± 75.00 µg g−1 DW, whereas the total concentration of the leaf extracts of non-transgenic control samples after glyphosate treatment was 3778.28 ± 59.73 µg g−1 DW. Major phenolic compounds that increased in the transgenic C. lanceolata plants after glyphosate treatment included kaempherol, gallic acid, myricetin, p-hydroxybenzjoic acid, quercetin, salicylic acid, t-cinnamic acid, catechin, benzoicacid, ferulic acid, protocatechuic acid, veratric acid, and vanillic acid. Among these, vanillic acid showed the greatest increase in both leaf and root extracts from transgenic plants relative to those from control C. lanceolata plants following treatment with glyphosate, which could affect the 5-enol-pyruvyl shikimate-3-phosphate (EPSP) synthase, an enzyme in the shikimate pathway. We observed enhanced stomatal conductance (gs) and photosynthesis rate (A) in the transgenic plants treated with water stress and glyphosate treatment. The results of this study demonstrated large variations in the functioning of secondary metabolites pathway in response glyphosate and water stress in transgenic C. lanceolata.
机译：研究了水分胁迫和除草剂处理对过量表达γ-tmt基因的党参党参植物中酚类化合物浓度和光合速率的影响，并与对照非转基因党参杉木植物进行了比较。在水分胁迫和使用草甘膦处理3周后，采用高效液相色谱结合二极管阵列检测技术在轮叶樟茶幼苗中研究了总酚类化合物的含量。在水分胁迫和草甘膦处理后，在转化的轮叶杉木植物的叶和根提取物中观察到酚类化合物组成的变化。水分胁迫后转基因样品叶片提取物中酚类化合物的总浓度为3455.13±40.48至8695.00±45.44 µg g −1 干重（DW），而非转基因对照样品的叶提取物为5630.83±45.91μgg -1 DW。转基因叶片中水分胁迫后增加的主要酚类化合物是（+）儿茶素，苯甲酸，绿原酸，阿魏酸，没食子酸，芦丁，香草酸和维拉酸。草甘膦处理后的转基因样品叶片提取物中酚类化合物的总浓度范围为4744.37±81.81至12,051.02±75.00 µg g -1 DW，而非转基因叶片提取物的总浓度为草甘膦处理后的对照样品为3778.28±59.73 µg g -1 DW。草甘膦处理后在转基因杉木植物中增加的主要酚类化合物包括kaempherol，没食子酸，杨梅素，对羟基苯甲jo酸，槲皮素，水杨酸，叔肉桂酸，儿茶素，苯甲酸，阿魏酸，原儿茶酸，维甲酸和香草酸。其中，与草甘膦处理后，相对于对照的披针茅种植物相比，香草酸在转基因植物的叶和根提取物中均表现出最大的增加，这可能影响5-烯醇-丙酮酸sh草酸酯-3-磷酸（EPSP）合酶。 ，是iki草酸酯途径中的一种酶。我们观察到在用水胁迫和草甘膦处理的转基因植物中气孔导度（gs）和光合作用速率（A）提高。这项研究的结果表明，在转基因杉木中次生代谢产物途径的功能在草甘膦和水分胁迫的响应中存在很大差异。

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