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首页> 美国卫生研究院文献>Marine Drugs >High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium
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High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium

机译：在流程富水生长培养基上的平板光生物反应器中培养的来自拟南芥的高EPA生物质

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Nannochloropsis salina was grown on a mixture of standard growth media and pre-gasified industrial process water representing effluent from a local biogas plant. The study aimed to investigate the effects of enriched growth media and cultivation time on nutritional composition of Nannochloropsis salina biomass, with a focus on eicosapentaenoic acid (EPA). Variations in fatty acid composition, lipids, protein, amino acids, tocopherols and pigments were studied and results compared to algae cultivated on F/2 media as reference. Mixed growth media and process water enhanced the nutritional quality of Nannochloropsis salina in laboratory scale when compared to algae cultivated in standard F/2 medium. Data from laboratory scale translated to the large scale using a 4000 L flat panel photo-bioreactor system. The algae growth rate in winter conditions in Denmark was slow, but results revealed that large-scale cultivation of Nannochloropsis salina at these conditions could improve the nutritional properties such as EPA, tocopherol, protein and carotenoids compared to laboratory-scale cultivated microalgae. EPA reached 44.2% ± 2.30% of total fatty acids, and α-tocopherol reached 431 ± 28 µg/g of biomass dry weight after 21 days of cultivation. Variations in chemical compositions of Nannochloropsis salina were studied during the course of cultivation. Nannochloropsis salina can be presented as a good candidate for winter time cultivation in Denmark. The resulting biomass is a rich source of EPA and also a good source of protein (amino acids), tocopherols and carotenoids for potential use in aquaculture feed industry.
机译：Nannochloropsis盐沼在标准生长培养基和代表本地沼气厂废水的预气化工业过程水的混合物上生长。这项研究旨在调查丰富的生长培养基和培养时间对拟南芥盐分生物量营养成分的影响，重点是二十碳五烯酸（EPA）。研究了脂肪酸组成，脂质，蛋白质，氨基酸，生育酚和色素的变化，并将结果与​​在F / 2培养基上培养的藻类进行了比较。与标准F / 2培养基中培养的藻类相比，混合生长培养基和工艺用水在实验室规模上提高了拟南芥的营养品质。使用4000 L平板光生物反应器系统将实验室规模的数据转换为大规模数据。丹麦冬季条件下的藻类生长速度很慢，但结果表明，与实验室规模的微藻相比，在这种条件下大规模培养拟南芥盐藻可以改善EPA，生育酚，蛋白质和类胡萝卜素等营养特性。培养21天后，EPA达到总脂肪酸的44.2％±2.30％，α-生育酚达到431±28 µg / g生物质干重。在培养过程中研究了拟南芥盐分的化学组成的变化。 Nannochloropsis salina可以作为丹麦冬季栽培的良好选择。产生的生物质既是EPA的丰富来源，又是蛋白质（氨基酸），生育酚和类胡萝卜素的良好来源，可用于水产养殖饲料工业。

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