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首页> 美国卫生研究院文献>The ISME Journal >Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire
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Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire

机译：珊瑚病原体弧菌弧菌的基因组和蛋白质组学分析显示了多种毒力库

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Vibrio coralliilyticus has been implicated as an important pathogen of coral species worldwide. In this study, the nearly complete genome of Vibrio coralliilyticus strain P1 (LMG23696) was sequenced and proteases implicated in virulence of the strain were specifically investigated. The genome sequence of P1 (5 513 256 bp in size) consisted of 5222 coding sequences and 58 RNA genes (53 tRNAs and at least 5 rRNAs). Seventeen metalloprotease and effector (vgrG, hlyA and hcp) genes were identified in the genome and expressed proteases were also detected in the secretome of P1. As the VcpA zinc-metalloprotease has been considered an important virulence factor of V. coralliilyticus, a vcpA deletion mutant was constructed to evaluate the effect of this gene in animal pathogenesis. Both wild-type and mutant (ΔvcpA) strains exhibited similar virulence characteristics that resulted in high mortality in Artemia and Drosophila pathogenicity bioassays and strong photosystem II inactivation of the coral dinoflagellate endosymbiont (Symbiodinium). In contrast, the ΔvcpA mutant demonstrated higher hemolytic activity and secreted 18 proteins not secreted by the wild type. These proteins included four types of metalloproteases, a chitinase, a hemolysin-related protein RbmC, the Hcp protein and 12 hypothetical proteins. Overall, the results of this study indicate that V. coralliilyticus strain P1 has a diverse virulence repertoire that possibly enables this bacterium to be an efficient animal pathogen.
机译：溶珊瑚弧菌已被认为是全世界珊瑚物种的重要病原体。在这项研究中，对溶珊瑚弧菌菌株P1（LMG23696）的近乎完整的基因组进行了测序，并专门研究了与该菌株毒力有关的蛋白酶。 P1的基因组序列（大小为5 513 256 bp）由5222个编码序列和58个RNA基因（53个tRNA和至少5个rRNA）组成。在基因组中鉴定出十七个金属蛋白酶和效应基因（vgrG，hlyA和hcp），并且在P1的分泌组中也检测到表达的蛋白酶。由于VcpA锌金属蛋白酶被认为是溶血弧菌的重要毒力因子，因此构建了vcpA缺失突变体来评估该基因在动物发病机理中的作用。野生型和突变型（ΔvcpA）菌株均显示出相似的毒力特征，导致卤虫和果蝇致病性生物测定中的高死亡率，以及珊瑚鞭毛鞭毛内共生体（Symbiodinium）的强光系统II失活。相反，ΔvcpA突变体显示出更高的溶血活性，并分泌了18种非野生型分泌的蛋白质。这些蛋白质包括四种类型的金属蛋白酶，几丁质酶，溶血素相关蛋白RbmC，Hcp蛋白和12种假设的蛋白。总体而言，这项研究的结果表明，溶血弧菌菌株P1具有多种毒力库，可能使这种细菌成为有效的动物病原体。

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