• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Marine Drugs >Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1
【2h】

Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

机译：铜藻假单胞菌稳定的金纳米粒子介导的生物膜抑制铜绿假单胞菌PAO1的毒力和运动特性的衰减。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The emergence of antibiotic resistance in Pseudomonas aeruginosa due to biofilm formation has transformed this opportunistic pathogen into a life-threatening one. Biosynthesized nanoparticles are increasingly being recognized as an effective anti-biofilm strategy to counter P. aeruginosa biofilms. In the present study, gold nanoparticles (AuNPs) were biologically synthesized and stabilized using fucoidan, which is an active compound sourced from brown seaweed. Biosynthesized fucoidan-stabilized AuNPs (F-AuNPs) were subjected to characterization using UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission transmission electron microscopy (FE-TEM), dynamic light scattering (DLS), and energy dispersive X-ray diffraction (EDX). The biosynthesized F-AuNPs were then evaluated for their inhibitory effects on P. aeruginosa bacterial growth, biofilm formation, virulence factor production, and bacterial motility. Overall, the activities of F-AuNPs towards P. aeruginosa were varied depending on their concentration. At minimum inhibitory concentration (MIC) (512 µg/mL) and at concentrations above MIC, F-AuNPs exerted antibacterial activity. In contrast, the sub-inhibitory concentration (sub-MIC) levels of F-AuNPs inhibited biofilm formation without affecting bacterial growth, and eradicated matured biofilm. The minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were identified as 128 µg/mL. Furthermore, sub-MICs of F-AuNPs also attenuated the production of several important virulence factors and impaired bacterial swarming, swimming, and twitching motilities. Findings from the present study provide important insights into the potential of F-AuNPs as an effective new drug for controlling P. aeruginosa-biofilm-related infections.
机译：铜绿假单胞菌由于生物膜形成而产生的抗生素抗性已将这种机会病原体转化为威胁生命的病原体。生物合成的纳米颗粒正日益被认为是对抗铜绿假单胞菌生物膜的有效抗生物膜策略。在本研究中，金纳米颗粒（AuNPs）是由岩藻依聚糖生物合成和稳定的，岩藻依聚糖是一种来自褐藻的活性化合物。使用紫外可见光谱，傅里叶变换红外光谱（FTIR），场发射透射电子显微镜（FE-TEM），动态光散射（DLS）和能量色散X对生物合成的岩藻依聚糖稳定化的AuNP（F-AuNPs）进行表征射线衍射（EDX）。然后评估生物合成的F-AuNPs对铜绿假单胞菌细菌生长，生物膜形成，毒力因子产生和细菌运动性的抑制作用。总体而言，F-AuNP对铜绿假单胞菌的活性随浓度而变化。在最低抑菌浓度（MIC）（512 µg / mL）和高于MIC的浓度下，F-AuNPs发挥抗菌活性。相比之下，F-AuNPs的亚抑制浓度（sub-MIC）水平抑制了生物膜的形成而不影响细菌的生长，并消除了成熟的生物膜。最小生物膜抑制浓度（MBIC）和最小生物膜清除浓度（MBEC）为128 µg / mL。此外，F-AuNP的亚MIC还减弱了几种重要毒力因子的产生，并削弱了细菌群，游泳和抽搐的动力。本研究的发现为F-AuNPs作为控制铜绿假单胞菌生物膜相关感染的有效新药的潜力提供了重要的见识。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号