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首页> 美国卫生研究院文献>Iranian Journal of Pharmaceutical Research : IJPR >Down-Regulation of Glycosyl Transferase Genes in Streptococcus Mutans by Punica Granatum L. Flower and Rhus Coriaria L. Fruit Water Extracts
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Down-Regulation of Glycosyl Transferase Genes in Streptococcus Mutans by Punica Granatum L. Flower and Rhus Coriaria L. Fruit Water Extracts

机译：石榴籽花和大花i果实水提取物对变形链球菌中糖基转移酶基因的下调

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摘要

In our previous studies, we showed the inhibitory effects of Punica granatum L. flower and Rhus coriaria L. fruit water extracts on dental plaque accumulation by several bacteria, especially Streptococcus mutans (S. mutans), on orthodontic wire by in-vitro assays. In this study, the anti-cariogenic properties of the extracts were evaluated by assessing their effects on expression of glycosyltransferase (gtf) genes, which are responsible for initial biofilm formation by S. mutans.In this study, the effect of herbal extracts on expression of gtfB, C (encoding enzymes that produce water-insoluble glucans) and D (encoding enzymes that produce water-soluble glucans) genes in S. mutans growing in planktonic state was evaluated quantitatively by real-time polymerase chain reaction (PCR) method.The minimum biofilm inhibitory concentration (MBIC) of understudied herbal water extracts significantly suppressed gtfB, C and D gene expression by 85.3 ± 7.5%, 33.3 ± 6.4% and 25 ± 14%, respectively for Punica granatum L. extract and 73.4 ± 7.3%, 93.8 ± 2.7% and 59.3 ± 9.8%, respectively for Rhus coriaria L. extract compared to the non-treated control group (P < 0.05). Also, the real-rime PCR showed that the inhibitory effect of Rhus coriaria L. extract on gtfC and D was significantly greater (10.8 and 1.8 fold, respectively) than that of Punica granatum L. extract.These findings suggest that Punica granatum L. and especially Rhus coriaria L. maybe used as novel, natural antiplaque agents since they inhibit specific genes associated with bacterial biofilm formation without necessarily affecting the growth of oral bacteria.
机译：在我们以前的研究中，我们通过体外试验显示了石榴皮花和大叶红景天果实水提取物对几种细菌（尤其是变形链球菌）对正畸丝的牙菌斑积累的抑制作用。在这项研究中，通过评估提取物对糖基转移酶（gtf）基因表达的影响来评估其提取物的抗龋齿特性，该基因是由变形链球菌形成初始生物膜的原因。通过实时聚合酶链反应（PCR）方法定量评估gtfB中浮游状态生长的变形链球菌中C（产生不溶于水的葡聚糖的编码酶）和D（产生水溶性葡聚糖的编码酶）基因。被研究的草药水提取物的最低生物膜抑制浓度（MBIC）分别对石榴皮提取物和73.4±7.3％分别显着抑制gtfB，C和D基因表达85.3±7.5％，33.3±6.4％和25±14％。与未经处理的对照组相比，Rusus coriaria L.提取物分别为93.8±2.7％和59.3±9.8％（P <0.05）。另外，真实框PCR显示出对Rhus coriaria Lem的抑制作用。 gtfC 和 D 上的提取物显着大于 Punica granatum L的（分别为10.8和1.8倍）。这些发现表明 Punica granatum L。尤其是 Rhus coriaria L 。 可以用作新型天然抗斑剂，因为它们可以抑制与细菌生物膜形成相关的特定基因，而不必影响口腔细菌的生长。

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