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Phylogeny and species delimitations in the entomopathogenic genus Beauveria (Hypocreales Ascomycota) including the description of B.peruviensis sp. nov.

机译:昆虫致病性白僵菌属(HypocrealesAscomycota)的系统发育和物种划分包括B的描述。秘鲁藻十一月

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摘要

The genus Beauveria is considered a cosmopolitan anamorphic and teleomorphic genus of soilborne necrotrophic arthropod-pathogenic fungi that includes ecologically and economically important species. Species identification in Beauveria is difficult because of its structural simplicity and the lack of distinctive phenotypic variation. Therefore, the use of multi-locus sequence data is essential to establish robust species boundaries in addition to DNA-based species delimitation methods using genetic distance, coalescent, and genealogical concordance approaches (polyphasic approaches). In this regard, our study used multilocus phylogeny and five DNA-based methods to delimit species in Beauveria using three molecular makers. These polyphasic analyses allowed for the delimitation of 20–28 species in Beauveria, confirming cryptic diversity in five species (i.e. B. amorpha, B. bassiana, B. diapheromeriphila, and B. pseudobassiana) and supporting the description of B. peruviensis as a new taxon from northeastern Peru. The other five species were not evaluated as they did not have enough data (i.e. B. araneola, B. gryllotalpidicola, B. loeiensis, B. medogensis, and B. rudraprayagi). Our results demonstrate that the congruence among different methods in a polyphasic approach (e.g. genetic distance and coalescence methods) is more likely to show reliably supported species boundaries. Among the methods applied in this study, genetic distance, coalescent approaches, and multilocus phylogeny are crucial when establishing species boundaries in Beauveria.
机译:球孢白僵菌属被认为是土壤传播的坏死性节肢动物病原性真菌的世界性变态和远变形属,包括生态和经济上重要的物种。由于其结构简单和缺乏独特的表型变异,因此在白僵菌中很难进行物种鉴定。因此,除了使用遗传距离,合并和族谱一致性方法(多相方法)的基于DNA的物种定界方法外,使用多基因座序列数据对于建立稳固的物种边界至关重要。在这方面,我们的研究使用多基因组系统发育和五种基于DNA的方法,使用三个分子标记物对白僵菌进行定界。这些多相分析允许在白僵菌中定界20–28个物种,从而确认了5个物种(即紫穗槐, B)的隐性多样性。 bassiana B. < / em> diapheromeriphila B. < / em> pseudobassiana ),并支持 B。 peruviensis 作为秘鲁东北部的新分类群。其他五个物种没有足够的数据,因此未进行评估(即 B。 araneola B。 gryllotalpidicola B。 loeiensis B。 成岩作用< / em> B。 rudraprayagi < / em> )。我们的结果表明,在多相方法中不同方法之间的一致性(例如遗传距离和合并方法)更可能显示可靠支持的物种边界。在本研究中使用的方法中,在建立 Beauveria 的物种边界时,遗传距离,合并方法和多基因座系统发育至关重要。

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