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首页> 美国卫生研究院文献>International Journal of Nanomedicine >Synthesis and application of luminescent single CdS quantum dot encapsulated silica nanoparticles directed for precision optical bioimaging
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Synthesis and application of luminescent single CdS quantum dot encapsulated silica nanoparticles directed for precision optical bioimaging

机译：发光单CdS量子点包裹的二氧化硅纳米粒子的合成及其在精密光学生物成像中的应用

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This paper presents the synthesis of aqueous cadmium sulfide (CdS) quantum dots (QDs) and silica-encapsulated CdS QDs by reverse microemulsion method and utilized as targeted bio-optical probes. We report the role of CdS as an efficient cell tag with fluorescence on par with previously documented cadmium telluride and cadmium selenide QDs, which have been considered to impart high levels of toxicity. In this study, the toxicity of bare QDs was efficiently quenched by encapsulating them in a biocompatible coat of silica. The toxicity profile and uptake of bare CdS QDs and silica-coated QDs, along with the CD31-labeled, silica-coated CdS QDs on human umbilical vein endothelial cells and glioma cells, were investigated. The effect of size, along with the time-dependent cellular uptake of the nanomaterials, has also been emphasized. Enhanced, high-specificity imaging toward endothelial cell lines in comparison with glioma cells was achieved with CD31 antibody-conjugated nanoparticles. The silica-coated nanomaterials exhibited excellent biocompatibility and greater photostability inside live cells, in addition to possessing an extended shelf life. In vivo biocompatibility and localization study of silica-coated CdS QDs in medaka fish embryos, following direct nanoparticle exposure for 24 hours, authenticated the nanomaterials’ high potential for in vivo imaging, augmented with superior biocompatibility. As expected, CdS QD-treated embryos showed 100% mortality, whereas the silica-coated QD-treated embryos stayed viable and healthy throughout and after the experiments, devoid of any deformities. We provide highly cogent and convincing evidence for such silica-coated QDs as a model nanoparticle in practice, to achieve in vitro and in vivo precision targeted imaging.
机译：本文介绍了通过反向微乳液法合成硫化镉（CdS）量子点（QDs）和二氧化硅包裹的CdS QDs并用作目标生物光学探针。我们报道CdS作为具有荧光的高效细胞标签的作用，与以前记录的碲化镉和硒化镉QD相当，后者被认为具有很高的毒性。在这项研究中，将裸露的量子点封装在生物相容性二氧化硅涂层中，可以有效地消除其毒性。研究了裸露的CdS QD和二氧化硅包被的QD以及CD31标记的二氧化硅包被的CdS QD对人脐静脉内皮细胞和神经胶质瘤细胞的毒性和摄取情况。还强调了尺寸的影响以及纳米材料随时间的细胞吸收。与神经胶质瘤细胞相比，用CD31抗体偶联的纳米颗粒实现了对内皮细胞系的增强，高特异性成像。二氧化硅涂覆的纳米材料除了具有延长的保存期限外，还表现出优异的生物相容性和在活细胞内部的更高的光稳定性。在直接暴露于纳米粒子24小时之后，鱼鱼胚中的二氧化硅包覆的CdS QD的体内生物相容性和定位研究证实了纳米材料在体内成像方面的巨大潜力，并具有优越的生物相容性。正如预期的那样，经CdS QD处理的胚胎显示出100％的死亡率，而经二氧化硅包覆的QD处理的胚胎在整个实验过程中和之后均保持活力和健康，没有任何畸变。在实践中，我们为此类二氧化硅包覆的量子点作为模型纳米粒子提供了非常有说服力和令人信服的证据，以实现体外和体内精确的靶向成像。

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