• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>International Journal of Nanomedicine >Different patterns of nuclear and mitochondrial penetration by the G3 PAMAM dendrimer and its biotin–pyridoxal bioconjugate BC-PAMAM in normal and cancer cells in vitro
【2h】

Different patterns of nuclear and mitochondrial penetration by the G3 PAMAM dendrimer and its biotin–pyridoxal bioconjugate BC-PAMAM in normal and cancer cells in vitro

机译：G3 PAMAM树状大分子及其生物素-吡py醛生物缀合物BC-PAMAM在正常细胞和癌细胞中的核和线粒体穿透方式不同

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The intracellular localization and colocalization of a fluorescently labeled G3 amine-terminated cationic polyamidoamine (PAMAM) dendrimer and its biotin–pyridoxal (BC-PAMAM) bioconjugate were investigated in a concentration-dependent manner in normal human fibroblast (BJ) and squamous epithelial carcinoma (SCC-15) cell lines. After 24 hours treatment, both cell lines revealed different patterns of intracellular dendrimer accumulation depending on their cytotoxic effects. Cancer cells exhibited much higher (20-fold) tolerance for native PAMAM treatment than fibroblasts, whereas BC-PAMAM was significantly toxic only for fibroblasts at 50 µM concentration. Fibroblasts accumulated the native and bioconjugated dendrimers in a concentration-dependent manner at nontoxic range of concentration, with significantly lower bioconjugate loading. After reaching the cytotoxicity level, fluorescein isothiocyanate-PAMAM accumulation remains at high, comparable level. In cancer cells, native PAMAM loading at higher, but not cytotoxic concentrations, was kept at constant level with a sharp increase at toxic concentration. Mander’s coefficient calculated for fibroblasts and cancer cells confirmed more efficient native PAMAM penetration as compared to BC-PAMAM. Significant differences in nuclear dendrimer penetration were observed for both cell lines. In cancer cells, PAMAM signals amounted to ~25%–35% of the total nuclei area at all investigated concentrations, with lower level (15%–25%) observed for BC-PAMAM. In fibroblasts, the dendrimer nuclear signal amounted to 15% at nontoxic and up to 70% at toxic concentrations, whereas BC-PAMAM remained at a lower concentration-dependent level (0.3%–20%). Mitochondrial localization of PAMAM and BC-PAMAM revealed similar patterns in both cell lines, depending on the extracellular dendrimer concentration, and presented significantly lower signals from BC-PAMAM, which correlated well with the cytotoxicity.
机译：在正常人成纤维细胞（BJ）和鳞状上皮癌（BJ）中，以浓度依赖的方式研究了荧光标记的G3胺基端接的阳离子聚酰胺型胺（PAMAM）树状大分子及其生物素-吡rid醛（BC-PAMAM）生物缀合物的细胞内定位和共定位。 SCC-15）细胞系。处理24小时后，两种细胞系均显示出不同的细胞内树状大分子蓄积模式，这取决于它们的细胞毒性作用。癌细胞对天然PAMAM处理的耐受性比成纤维细胞高（20倍），而BC-PAMAM仅对浓度为50 µM的成纤维细胞有明显毒性。成纤维细胞以浓度依赖性方式在无毒浓度范围内积累天然和生物共轭的树状大分子，生物共轭物的负载明显降低。达到细胞毒性水平后，异硫氰酸荧光素-PAMAM积累保持在较高的可比水平。在癌细胞中，天然PAMAM在较高浓度而不是细胞毒性浓度下保持恒定水平，并且毒性浓度急剧增加。根据成纤维细胞和癌细胞计算出的Mander系数证实，与BC-PAMAM相比，天然PAMAM的渗透效率更高。观察到两种细胞系的核树状大分子渗透的显着差异。在癌细胞中，在所有研究浓度下，PAMAM信号占总细胞核面积的〜25％–35％，而BC-PAMAM的水平较低（15％–25％）。在成纤维细胞中，树状聚合物核信号在无毒时达15％，在有毒浓度时达70％，而BC-PAMAM则保持在较低的浓度依赖性水平（0.3％–20％）。 PAMAM和BC-PAMAM的线粒体定位在两种细胞系中显示出相似的模式，具体取决于细胞外树状大分子的浓度，并且呈现出明显更低的BC-PAMAM信号，这与细胞毒性密切相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号