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首页> 美国卫生研究院文献>International Journal of Nanomedicine >Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation
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Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation

机译：创新的可生物降解的聚（L-丙交酯）/胶原蛋白/羟基磷灰石复合纤维支架促进成骨细胞的增殖和分化

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The development of an artificial bone graft which can promote the regeneration of fractures or diseased bones is currently the most challenging aspect in bone tissue engineering. To achieve the purpose of promoting bone proliferation and differentiation, the artificial graft needs have a similar structure and composition of extracellular matrix. One-step electrospinning method of biocomposite nanofibers containing hydroxyapatite (HA) nanoparticles and collagen (Coll) were developed for potential application in bone tissue engineering. Nanocomposite scaffolds of poly(L-lactide) (PLLA), PLLA/HA, PLLA/Coll, and PLLA/Coll/HA were fabricated by electrospinning. The morphology, diameter, elements, hydrophilicity, and biodegradability of the composite scaffolds have been investigated. The biocompatibility of different nanocomposite scaffolds was assessed using mouse osteoblasts MC3T3-E1 in vitro, and the proliferation, differentiation, and mineralization of cells on different nanofibrous scaffolds were investigated. The results showed that PLLA/Coll/HA nanofiber scaffolds enhanced cell adhesion, spreading, proliferation, differentiation, mineralization, and gene expression of osteogenic markers compared to other scaffolds. In addition, the nanofibrous scaffolds maintained a stable composition at the beginning of the degradation period and morphology wastage and weight loss were observed when incubated for up to 80 days in physiological simulated conditions. The PLLA/Coll/HA composite nanofibrous scaffolds could be a potential material for guided bone regeneration.
机译：目前，可促进骨折或患病骨骼再生的人造骨移植物的开发是骨组织工程中最具挑战性的方面。为了达到促进骨增殖和分化的目的，人造移植物需要具有相似的细胞外基质的结构和组成。开发了一种包含羟基磷灰石（HA）纳米颗粒和胶原蛋白（Coll）的生物复合纳米纤维的一步静电纺丝方法，有望在骨组织工程中应用。通过电纺丝制备聚（L-丙交酯）（PLLA），PLLA / HA，PLLA / Coll和PLLA / Coll / HA的纳米复合支架。研究了复合支架的形态，直径，元素，亲水性和生物降解性。使用小鼠成骨细胞MC3T3-E1在体外评估了不同纳米复合材料支架的生物相容性，并研究了不同纳米纤维支架上细胞的增殖，分化和矿化。结果表明，与其他支架相比，PLLA / Coll / HA纳米纤维支架增强了成骨标记的细胞粘附，扩散，增殖，分化，矿化和基因表达。另外，纳米纤维支架在降解期开始时保持稳定的组成，并且在生理模拟条件下孵育长达80天时观察到形态浪费和重量减轻。 PLLA / Coll / HA复合纳米纤维支架可能是引导骨再生的潜在材料。

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