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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae) an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China
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Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae) an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China

机译：中国西南金沙江干热河谷特有濒危植物木槿（木槿科）的微卫星基因座的分离与鉴定

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Hibiscus aridicola (Malvaceae) is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-streptavidin capture method, a total of 40 microsatellite markers were developed and characterized in H. aridicola for the first time. Polymorphisms were evaluated in 39 individuals from four natural populations. Fifteen of the markers showed polymorphisms with two to six alleles per locus; the observed heterozygosity ranged from 0.19 to 0.72. These microsatellite loci would be useful tools for population genetics studies on H. aridicola and other con-generic species which are important to the conservation and development of endangered species.
机译：芙蓉（锦葵科）是中国西南部金沙江干热河谷特有的濒危观赏灌木。根据我们的现场调查，野外仅存在4个自然的H. aridicola种群。可以推断，H。aridicola在野生环境中面临极高的灭绝风险，因此需要紧急的保护策略。通过使用改良的生物素-链霉亲和素捕获方法，首次开发了40种微卫星标记物并在H. aridicola中进行了表征。在来自四个自然种群的39个个体中评估了多态性。十五个标记显示出多态性，每个基因座具有两个至六个等位基因。观察到的杂合度为0.19至0.72。这些微卫星基因座将是对H. aridicola和其他同属物种进行种群遗传学研究的有用工具，这对于保护和发展濒危物种至关重要。

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