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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Early Diagnosis of Irkut Virus Infection Using Magnetic Bead-Based Serum Peptide Profiling by MALDI-TOF MS in a Mouse Model
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Early Diagnosis of Irkut Virus Infection Using Magnetic Bead-Based Serum Peptide Profiling by MALDI-TOF MS in a Mouse Model

机译：MALDI-TOF MS在小鼠模型中使用基于磁珠的血清肽谱分析对伊尔库特病毒感染的早期诊断

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Early diagnosis is important for the prompt post-exposure prophylaxis of lyssavirus infections. To diagnose Irkut virus (IRKV) infection during incubation in mice, a novel method using magnetic bead-based serum peptide profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been established. For this test, serum peptides were concentrated by adsorption to and elution from the magnetic bead-based weak cation ion exchanger. Mass spectrograms obtained by MALDI-TOF MS were analyzed using ClinProTools bioinformatics software. Construction of the diagnostic model was performed using serum samples from mice infected with IRKV and rabies virus (RABV) BD06, Flury-LEP, and SRV9 (as controls). The method accurately diagnosed sera 2, 4 and 8 days after IRKV and RABV infections. The sensitivity, specificity, and total accuracy of diagnosis were 86.7%, 95.2%, and 92.9%, respectively. However, IRKV could not be differentiated from RABV 1 day after infection. The results of the present study indicate that serum peptide profiling by MALDI-TOF MS is a promising technique for the early clinical diagnosis of lyssavirus infections and needs to be further tested in humans and farm animals.
机译：早期诊断对迅速暴露后预防狂犬病病毒感染很重要。为了在小鼠孵育过程中诊断出伊尔库特病毒（IRKV）感染，已建立了一种新的方法，该方法使用了基于磁珠的血清肽谱，并通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）进行了分析。对于该测试，通过吸附至磁珠基弱阳离子交换剂并从中洗脱来浓缩血清肽。使用ClinProTools生物信息学软件分析通过MALDI-TOF MS获得的质谱图。使用感染了IRKV和狂犬病毒（RABV）BD06，Flury-LEP和SRV9的小鼠的血清样品（作为对照）构建诊断模型。该方法可在IRKV和RABV感染后第2、4和8天准确诊断出血清。诊断的敏感性，特异性和总准确性分别为86.7％，95.2％和92.9％。但是，感染后1天无法将IRKV与RABV区分。本研究的结果表明，通过MALDI-TOF MS进行血清肽谱分析是一种对狂犬病病毒感染进行早期临床诊断的有前途的技术，需要在人和农场动物中进行进一步的测试。

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