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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Metal Free Graphene Oxide (GO) Nanosheets and Pristine-Single Wall Carbon Nanotubes (p-SWCNTs) Biocompatibility Investigation: A Comparative Study in Different Human Cell Lines
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Metal Free Graphene Oxide (GO) Nanosheets and Pristine-Single Wall Carbon Nanotubes (p-SWCNTs) Biocompatibility Investigation: A Comparative Study in Different Human Cell Lines

机译：无金属氧化石墨烯（GO）纳米片和原始的单壁碳纳米管（p-SWCNTs）生物相容性研究：在不同的人类细胞系中的比较研究。

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The in vitro biocompatibility of Graphene Oxide (GO) nanosheets, which were obtained by the electrochemical exfoliation of graphite electrodes in an electrolytic bath containing salts, was compared with the pristine Single Wall Carbon Nanotubes (p-SWCNTs) under the same experimental conditions in different human cell lines. The cells were treated with different concentrations of GO and SWCNTs for up to 48 h. GO did not induce any significant morphological or functional modifications (demonstrating a high biocompatibility), while SWNCTs were toxic at any concentration used after a few hours of treatment. The cell viability or cytotoxicity were detected by the trypan blue assay and the lactate dehydrogenase LDH quantitative enzymatic test. The Confocal Laser Scanning Microscopy (CLSM) and transmission electron microscopy (TEM) analysis demonstrated the uptake and internalization of GO sheets into cells, which was localized mainly in the cytoplasm. Different results were observed in the same cell lines treated with p-SWCNTs. TEM and CLSM (Confocal Laser Scanning Microscopy) showed that the p-SWCNTs induced vacuolization in the cytoplasm, disruption of cellular architecture and damage to the nuclei. The most important result of this study is our finding of a higher GO biocompatibility compared to the p-SWCNTs in the same cell lines. This means that GO nanosheets, which are obtained by the electrochemical exfoliation of a graphite-based electrode (carried out in saline solutions or other physiological working media) could represent an eligible nanocarrier for drug delivery, gene transfection and molecular cell imaging tests.
机译：通过在含盐的电解浴中对石墨电极进行电化学剥离而获得的氧化石墨烯（GO）纳米片的体外生物相容性与原始的单壁碳纳米管（p-SWCNTs）在相同的实验条件下进行了比较人类细胞系。用不同浓度的GO和SWCNT处理细胞长达48小时。 GO不会引起任何明显的形态或功能修饰（表现出高的生物相容性），而SWNCT在治疗数小时后，无论以任何浓度使用，均具有毒性。通过锥虫蓝试验和乳酸脱氢酶LDH定量酶试验检测细胞活力或细胞毒性。共聚焦激光扫描显微镜（CLSM）和透射电子显微镜（TEM）分析表明GO片被细胞吸收和内化，而这些细胞主要定位在细胞质中。在用p-SWCNT处理的相同细胞系中观察到不同的结果。 TEM和CLSM（Confocal激光扫描显微镜）显示，p-SWCNTs诱导细胞质中的空泡化，细胞结构的破坏和细胞核的破坏。这项研究最重要的结果是我们发现与相同细胞系中的p-SWCNT相比，GO生物相容性更高。这意味着，通过石墨基电极的电化学剥离（在盐溶液或其他生理工作介质中进行）获得的GO纳米片可以代表用于药物输送，基因转染和分子细胞成像测试的合格纳米载体。

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