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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Genome-Wide Identification and Analysis of Apple NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER Family (NPF) Genes Reveals MdNPF6.5 Confers High Capacity for Nitrogen Uptake under Low-Nitrogen Conditions
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Genome-Wide Identification and Analysis of Apple NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER Family (NPF) Genes Reveals MdNPF6.5 Confers High Capacity for Nitrogen Uptake under Low-Nitrogen Conditions

机译：苹果硝酸盐转运蛋白1 /肽转运蛋白家族（NPF）基因的全基因组鉴定和分析揭示了MdNPF6.5赋予低氮条件下高氮素吸收能力

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The NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family (NPF) proteins play important roles in moving substrates such as nitrate, peptides, amino acids, dicarboxylates, malate, glucosinolates, indole acetic acid (IAA), abscisic acid (ABA), and jasmonic acid. Although a unified nomenclature of NPF members in plants has been reported, this gene family has not been studied as thoroughly in apple (Malus × domestica Borkh.) as it has in other species. Our objective was to provide general information about apple MdNPFs and analyze the transcriptional responses of some members to different levels of nitrate supplies. We identified 73 of these genes from the apple genome and used phylogenetic analysis to organize them into eight major groups. These apple NPFs are structurally conserved, based on alignment of amino acid sequences and analyses of phylogenetics and conserved domains. Examination of their genomic structures indicated that these genes are highly conserved among other species. We monitored 14 cloned MdNPFs that showed varied expression patterns under different nitrate concentrations and in different tissues. Among them, NPF6.5 was significantly induced by both low and high levels of nitrate. When compared with the wild type, 35S:MdNPF6.5 transgenic apple calli were more tolerant to low-N stress, which demonstrated that this gene confers greater capacity for nitrogen uptake under those conditions. We also analyzed the expression patterns of those 73 genes in various tissues. Our findings benefit future research on this family of genes.
机译：硝酸盐转运蛋白1 /肽转运蛋白家族（NPF）蛋白在移动底物（如硝酸盐，肽，氨基酸，二羧酸盐，苹果酸盐，芥子油苷，吲哚乙酸（IAA），脱落酸（ABA）和茉莉酸）中起重要作用。尽管已经报道了植物中NPF成员的统一命名法，但是在苹果（Malus×domestica Borkh。）中，该基因家族尚未像在其他物种中那样被彻底研究。我们的目标是提供有关苹果MdNPF的一般信息，并分析某些成员对不同水平硝酸盐供应的转录反应。我们从苹果基因组中鉴定出这些基因中的73个，并使用系统发育分析将它们分为八个主要组。根据氨基酸序列的比对以及系统发育和保守域的分析，这些苹果NPF在结构上是保守的。检查它们的基因组结构表明这些基因在其他物种中是高度保守的。我们监测了14个克隆的MdNPF，它们在不同硝酸盐浓度和不同组织中显示出不同的表达模式。其中，低水平和高水平硝酸盐均显着诱导了NPF6.5。与野生型相比，转基因35S：MdNPF6.5苹果愈伤组织对低氮胁迫耐受性更高，这表明该基因在这些条件下具有更大的氮吸收能力。我们还分析了这73个基因在各种组织中的表达模式。我们的发现有益于对该基因家族的未来研究。

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