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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Assessment of Genetic Diversity Population Structure and Evolutionary Relationship of Uncharacterized Genes in a Novel Germplasm Collection of Diploid and Allotetraploid Gossypium Accessions Using EST and Genomic SSR Markers
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Assessment of Genetic Diversity Population Structure and Evolutionary Relationship of Uncharacterized Genes in a Novel Germplasm Collection of Diploid and Allotetraploid Gossypium Accessions Using EST and Genomic SSR Markers

机译：利用EST和基因组SSR标记评估二倍体和异源四倍体棉种的新型种质资源中遗传多样性种群结构和未鉴定基因的进化关系

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This study evaluated the genetic diversity and population structures in a novel cotton germplasm collection comprising 132 diploids, including Glossypium klotzschianum and allotetraploid cotton accessions, including Glossypium barbadense, Glossypium darwinii, Glossypium tomentosum, Glossypium ekmanianum, and Glossypium stephensii, from Santa Cruz, Isabella, San Cristobal, Hawaiian, Dominican Republic, and Wake Atoll islands. A total of 111 expressed sequence tag (EST) and genomic simple sequence repeat (gSSR) markers produced 382 polymorphic loci with an average of 3.44 polymorphic alleles per SSR marker. Polymorphism information content values counted 0.08 to 0.82 with an average of 0.56. Analysis of a genetic distance matrix revealed values of 0.003 to 0.53 with an average of 0.33 in the wild cotton collection. Phylogenetic analysis supported the subgroups identified by STRUCTURE and corresponds well with the results of principal coordinate analysis with a cumulative variation of 45.65%. A total of 123 unique alleles were observed among all accessions and 31 identified only in G. ekmanianum. Analysis of molecular variance revealed highly significant variation between the six groups identified by structure analysis with 49% of the total variation and 51% of the variation was due to diversity within the groups. The highest genetic differentiation among tetraploid populations was observed between accessions from the Hawaiian and Santa Cruz regions with a pairwise FST of 0.752 (p < 0.001). DUF819 containing an uncharacterized gene named yjcL linked to genomic markers has been found to be highly related to tryptophan-aspartic acid (W-D) repeats in a superfamily of genes. The RNA sequence expression data of the yjcL-linked gene Gh_A09G2500 was found to be upregulated under drought and salt stress conditions. The existence of genetic diversity, characterization of genes and variation in novel germplasm collection will be a landmark addition to the genetic study of cotton germplasm.
机译：这项研究评估了一个新的棉花种质集合的遗传多样性和种群结构，该集合包括132个二倍体，包括Glossypium klotzschianum和异源四倍体棉花种质，包括Glossypium barbadense，Glossypium darwinii，Glossypium tomentosum，Glossypium ekmanianum和Glossypium stephensii，圣克里斯托瓦尔，夏威夷，多米尼加共和国和维克环礁群岛。总共111个表达的序列标签（EST）和基因组简单序列重复（gSSR）标记产生了382个多态位点，每个SSR标记平均有3.44个多态等位基因。多态信息含量值统计为0.08至0.82，平均值为0.56。遗传距离矩阵的分析显示，野生棉花采集中的值在0.003至0.53之间，平均值为0.33。系统发育分析支持由结构识别的亚组，并且与主坐标分析的结果非常吻合，累积变异为45.65％。在所有种质中共观察到123个独特的等位基因，仅在G. ekmanianum中鉴定出31个。分子变异分析显示，通过结构分析确定的六组之间的变异非常显着，其中总变异的49％和51％的变异归因于各组之间的多样性。在四倍体种群之间的遗传分化最高的是夏威夷和圣克鲁斯地区的种质，成对FST为0.752（p <0.001）。已经发现包含与基因组标记物连接的名为yjcL的未表征基因的DUF819与基因超家族中的色氨酸-天冬氨酸（W-D）重复序列高度相关。发现在干旱和盐胁迫条件下，yjcL连锁基因Gh_A09G2500的RNA序列表达数据上调。遗传多样性的存在，基因特征的鉴定以及新种质收集中的变异将是棉花种质遗传研究的重要里程碑。

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